Proteome Profiler Arrays

Greta J. Wegner, David J. Finkel, Amy E. James and Richard A Krzyzek

Array Group, Assay Department, R&D Systems

Feb 15, 2013

This video protocol provides step-by-step instructions for measuring the relative levels of receptor tyrosine kinase (RTK) phosphorylation in a single sample of cell lysates using the Proteome Profiler™ Human Phospho-RTK Antibody Array.

Data Examples

Figure 1. The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates.

Data Examples

Figure 1. The Human Angiogenesis Array detects multiple analytes in cell culture supernates.

Data Examples

Figure 1. The Human Adipokine Array detects multiple analytes in cell culture supernates. Cells were either untreated or treated as indicated below. Each array was incubated with 500 µL of cell culture supernate. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.