Human Fc epsilon RI alpha Antibody

Catalog #: AF6678 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6678
AF6678-SP

Detection of Fc epsilon R1 alpha in Human Blood Granulocytes by Flow Cytometry.

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Human Fc epsilon RI alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc epsilon RI alpha in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc epsilon RI alpha
Val26-Gln205
Accession # NP_001992

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Fc epsilon R1 alpha in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Sheep Anti-Human Fce RIa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6678, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc epsilon RI alpha

The alpha subunit of the high affinity IgE receptor (Fc epsilon RI alpha or Fc epsilon RIA) is an IgE‑binding type I transmembrane glycoprotein of the multichain immune recognition (MIRR) family (1, 2). The receptor, Fc epsilon RI, is a tetrameric complex of one alpha, one beta and two gamma subunits ( alpha beta gamma ₂) on mast cells and basophils (1). An alternate trimeric form ( alpha gamma ₂) is expressed on human, but not rodent, mast cells, basophils, eosinophils and professional antigen presenting cells (3). While the gamma subunit is essential for expression of Fc epsilon RI alpha on the cell surface and for cell signaling, the beta subunit, when present, increases the halflife of the Fc epsilon RI complex on the cell surface (3, 4). An isoform of the beta subunit, beta T, blocks processing of the alpha subunit and its cell surface expression (2, 3, 5). Human Fc epsilon RI alpha cDNA encodes 257 amino acids (aa) including a 25 aa signal sequence, a 180 aa extracellular domain containing two Ig‑like domains that bind IgE and an endoplasmic reticulum retention motif, a 21 aa transmembrane domain with a charged amino acid (Asp219) that contributes to intracellular transport, and a 32 aa cytoplasmic sequence (1, 3, 6). Human Fc epsilon RI alpha shares 50‑62% aa sequence identity with mouse, rat, equine, ovine, bovine, porcine and canine Fc epsilon RI alpha. Binding of IgE alone increases surface expression of Fc epsilon RI, while crosslinking of IgE/Fc epsilon RI complexes by IgE ligands (allergens) initiates receptor internalization and signaling (2, 4, 5). Mast cell and basophil activation by IgE/Fc epsilon RI crosslinking causes degranulation, releasing histamine, leukotrienes, prostaglandins, and other mediators of immediate‑type and late‑phase allergic reactions. Circulating autoantibodies that crosslink Fc epsilon RI alpha are often found in patients with chronic urticaria (7). Fc epsilon RI on human antigen presenting cells mediates uptake and processing of allergens for presentation by class II MHC (2, 3). Fc epsilon RI expression on human DC and Langerhans cells is up‑regulated during allergic reactions (atopy) and correlates with serum IgE concentration (3).

References

Shimizu, A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1907.
Abramson, J. and I. Pecht (2007) Immunol. Rev. 217:231.
Kraft, S. and J-P. Kinet (2007) Nat. Rev. Immunol. 7:365.
Yamasaki, S. and T. Saito (2008) J. Pharmacol. Sci. 106:336.
Brenzovich, J. et al. (2009) J. Leukoc. Biol. 86:1351.
Cauvi, D.M. et al. (2006) J. Biol. Chem. 281:10448.
Kikuchi, Y. et al. (2001) J. Allergy Clin. Immunol. 107:1056.

Long Name

Fc epsilon Receptor I alpha

Entrez Gene IDs

2205 (Human); 14125 (Mouse); 25047 (Rat)

Alternate Names

Fc epsilon RI alpha; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; FCE1A; Fc-epsilon RI-alpha; FcepsilonRI alpha chain; FcepsilonRIalpha; FCER1A; FcERI; FceRIa; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immunoglobulin epsilon receptor subunit alpha; igE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide