Human beta-Catenin Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: beta-Catenin

beta -Catenin is a 94-96 kDa member of the beta -Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta -Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta -Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta -Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta -Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being  beta -Catenin and Ca⁺⁺/non-beta -Catenin based. It now appears they work in tandem, with Ca⁺⁺ mobilization facilitating the translocation of beta -Catenin across the nuclear membrane. Human and mouse full-length beta -Catenin shows 99% aa sequence identity.

Product Datasheets

Product Specific Notices

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Related Research Areas

• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Cancer Biomarkers
• Cancer Stem Cell Transcription Factors
• Cell to Cell Adhesion Disassembly During EMT
• Early Endodermal Lineage Markers
• Epithelial Cell Polarity
• Mesenchymal Cells and EMT
• Neural Stem Cell Markers
• Notch Pathway
• Primitive Endoderm Markers
• Stem Cell Transcription Factor Regulators
• Transcription Factors and Regulators
• Transcriptional Co-Activators
• Wnt Family
• Wnt Intracellular Signaling