Human BMP-10 Propeptide Biotinylated Antibody Summary
Ser20-Arg313
Accession # O95393
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-10
BMP-10, along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of sequence related TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1‑3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked non-glycosylated homodimers (4, 5). In transfectants, BMP-10 is secreted as a cleaved mature dimer, an uncleaved proform dimer, and an uncleaved proform monomer (4). The propeptide of human BMP-10 shares 82% amino acid sequence identity with mouse and rat proBMP-10 and 19%‑34% with the propeptides of human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (6‑8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the growth of embryonic cardiomyocytes (7, 9, 10). The BMP-10 mediated proliferation of these cells requires Notch signaling (11). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 overexpression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to induce signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (12).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
- Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
- Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
- David, L. et al. (2007) Blood 109:1953.
- Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
- Chen, H. et al. (2004) Development 131:2219.
- Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
- Srivastava, D. and E.N. Olson (2000) Nature 407:221.
- Pashmforoush, M. et al. (2004) Cell 117:373.
- Grego-Bessa, J. et al. (2007) Dev. Cell 12:415.
- Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
Product Datasheets
Citations for Human BMP-10 Propeptide Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10
Authors: J Guo, B Liu, M Thorikay, M Yu, X Li, Z Tong, RM Salmon, RJ Read, P Ten Dijke, NW Morrell, W Li
Nature Communications, 2022-05-03;13(1):2395.
Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Detection -
Reduced circulating BMP10 and BMP9 and elevated endoglin are associated with disease severity, decompensation and pulmonary vascular syndromes in patients with cirrhosis
Authors: NE Owen, GJ Alexander, S Sen, K Bunclark, G Polwarth, J Pepke-Zaba, AP Davenport, NW Morrell, PD Upton
EBioMedicine, 2020-05-23;56(0):102794.
Species: Human
Sample Types: Plasma
Applications: ELISA (detection) -
The Prodomain-bound Form of Bone Morphogenetic Protein 10 Is Biologically Active on Endothelial Cells*
Authors: He Jiang, Richard M. Salmon, Paul D. Upton, Zhenquan Wei, Aleksandra Lawera, Anthony P. Davenport et al.
Journal of Biological Chemistry
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Homozygous GDF2 nonsense mutations result in a loss of circulating BMP9 and BMP10 and are associated with either PAH or an “HHT‐like” syndrome in children
Authors: Joshua Hodgson, Lidia Ruiz‐Llorente, Jamie McDonald, Oliver Quarrell, Kelechi Ugonna, James Bentham et al.
Molecular Genetics & Genomic Medicine
FAQs
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