Home / BMP-4 / Human BMP-4 Biotinylated Antibody

Human BMP-4 Biotinylated Antibody

Catalog #: BAF757
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF757
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-4 in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human (rh) BMP-2 is observed and 10% cross-reactivity with rhBMP-5 and rhBMP-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BMP‑4 (Catalog # 314-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1‑5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide‑linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide‑linked heterodimer (7‑9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR‑IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13‑15).

 

References
  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human BMP-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. The Binding of the Bone Morphogenetic Protein Antagonist Gremlin to Kidney Heparan Sulfate: Such Binding is not Essential for BMP Antagonism
    Authors: Arnold Junior Tatsinkam
    Int. J. Biochem. Cell Biol, 2016-12-12;0(0):.
    Applications: Bioassay
  2. A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering.
    Authors: Mazaki T, Shiozaki Y, Yamane K, Yoshida A, Nakamura M, Yoshida Y, Zhou D, Kitajima T, Tanaka M, Ito Y, Ozaki T, Matsukawa A
    Sci Rep, 2014-03-25;4(0):4457.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human BMP-4 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human BMP-4 Biotinylated Antibody and earn rewards!

Have you used Human BMP-4 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review