Human BRCA1 N-Terminus Antibody

Catalog #: AF2210
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2210
AF2210-SP

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BRCA1 N-Terminus Antibody Summary

Species Reactivity

Human

Specificity

Detects human BRCA1 N-Terminus in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BRCA1 N-Terminus
Met1-Gly200
Accession # P38398

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

3 µg/500 µg cell lysate

U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BRCA1 in Human Breast Cancer Tissue. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human BRCA1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRCA1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRCA1

The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks, and serves as part of a scaffold for assembling other DNA damage response or repair factors.

Long Name

Breast Cancer 1

Entrez Gene IDs

672 (Human); 12189 (Mouse); 497672 (Rat)

Alternate Names

BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human BRCA1 N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Overactive BRCA1 Affects Presenilin 1 in Induced Pluripotent Stem Cell-Derived Neurons in Alzheimer's Disease
Authors: M Wezyk, A Szybinska, J Wojsiat, M Szczerba, K Day, H Ronnholm, M Kele, M Berdynski, B Peplonska, JP Fichna, J Ilkowski, M Styczynska, A Barczak, M Zboch, A Filipek-Gl, K Bojakowski, M Skrzypczak, K Ginalski, M Kabza, I Makalowska, M Barcikowsk, U Wojda, A Falk, C Zekanowski
J. Alzheimers Dis., 2018-01-01;62(1):175-202.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
BRCA1 mediates protein homeostasis through the ubiquitination of PERK and IRE1
Authors: Robert Hromas, Gayathri Srinivasan, Ming Yang, Aruna Jaiswal, Taylor A. Totterdale, Linda Phillips et al.
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