Human CCL2/JE/MCP‑1 Biotinylated Antibody Summary
Gln24-Thr99
Accession # P13500
Applications
Human CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CCL2/JE/MCP-1 by ELISA Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma -overexpressing microparticles (black bars) or no microparticles (white bars) for 4 hours before activation with LPS or PAM3CSK4 for 24 hours. Supernatants were collected and pro-inflammatory cytokines IL-8 (top), MCP-1 (bottom) were measured by ELISA. Dotted line represents baseline cytokine production from unactivated cells with no microparticle exposure. Mean values with standard errors represent one of at least 3 experiments. Two-way ANOVA with Tukey's multiple comparison post test was performed to determine statistical significance. * indicates (p<0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25426628), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human CCL2/JE/MCP-1 by ELISA Microparticle composition influences inflammatory cytokine production from activated monocytes.THP-1 cells (A) or CD14 sorted blood monocytes (B) were treated with control microparticles (grey bars) or PPAR gamma -overexpressing microparticles (black bars) or no microparticles (white bars) for 4 hours before activation with LPS or PAM3CSK4 for 24 hours. Supernatants were collected and pro-inflammatory cytokines IL-8 (top), MCP-1 (bottom) were measured by ELISA. Dotted line represents baseline cytokine production from unactivated cells with no microparticle exposure. Mean values with standard errors represent one of at least 3 experiments. Two-way ANOVA with Tukey's multiple comparison post test was performed to determine statistical significance. * indicates (p<0.05). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25426628), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
CCL2, also known as monocyte chemotactic and activating factor (MCAF), was initially purified independently by two groups based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene. The human CCL2 cDNA encodes a 99 amino acid residue precursor protein with a 23 residue hydrophobic signal peptide that is cleaved to generate the 76 residue mature protein. Natural CCL2 is heterogeneous in size due to the addition of O-linked carbohydrates and sialic acid residues. In addition to fibroblasts; tumor cells, smooth muscle cells, endothelial cells, and mononuclear phagocytes can also produce CCL2 either constitutively or upon stimulation by various stimuli. CCL2 is a member of the beta (C-C) subfamily of chemokines. The existence of MCP-2 and MCP-3 with 62% and 73% amino acid identity respectively, to CCL2 have been reported.
Consistent with it being a member of the chemokine beta family, CCL2 has been shown to chemoattract monocytes. In addition, it will also activate monocytes to be cytostatic for some human tumor cell lines; to increase cytosolic free calcium; to generate and release monocyte superoxide anions and to release monocyte lysosomal enzymes in vitro. CCL2 was reported to be capable of regulating adhesion molecule expression and cytokine production in human monocytes as well as chemoattracting, activating, and inducing histamine release from basophils. The biological roles played by CCL2 in a number of inflammatory and non-inflammatory disease states characterized by the accumulation of leukocytes at the site of the lesion, including atherosclerosis, delayed hypersensitivity reactions, etc., are being determined. CCL2 can bind to the C-C chemokine receptor-1 that also binds MIP-1 alpha, RANTES and MIP-1 beta. A specific receptor for CCL2 has also been cloned from THP-1 and MonoMac 6 cells.
Product Datasheets
Citations for Human CCL2/JE/MCP‑1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
21
Citations: Showing 1 - 10
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Nanoparticles of Lactiplantibacillus plantarum K8 Reduce Staphylococcus aureus Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation
Authors: Hong, J;Son, M;Sin, J;Kim, H;Chung, DK;
Nutrients
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens
Authors: Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al.
Frontiers in Immunology
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The Global Phosphorylation Landscape of SARS-CoV-2 Infection
Authors: Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al.
Cell
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Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
Mediators Inflamm., 2020-07-06;2020(0):6087109.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA -
Induction of Chemokines by Hepatitis C Virus Proteins: Synergy of the Core Protein with Interleukin-1 beta and Interferon-gamma in Liver Bystander Cells
Authors: Sara Abouelasrar Salama, Mieke Gouwy, Alexandra De Zutter, Noëmie Pörtner, Lotte Vanbrabant, Nele Berghmans et al.
Journal of Interferon & Cytokine Research
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Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells
Authors: ZM Bian, MG Field, SG Elner, JM Kahlenberg, VM Elner
Exp. Eye Res., 2018-02-16;170(0):29-39.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury
Authors: RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin
EBioMedicine, 2017-04-20;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
Sci Rep, 2016-07-25;6(0):29909.
Species: Human
Sample Types: Serum
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Microparticles engineered to highly express peroxisome proliferator-activated receptor-gamma decreased inflammatory mediator production and increased adhesion of recipient monocytes.
Authors: Sahler J, Woeller C, Phipps R
PLoS ONE, 2014-11-26;9(11):e113189.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
Ocular Fibroblast Diversity: Implications for Inflammation and Ocular Wound Healing
Authors: Xia Xi, David H. McMillan, Geniece M. Lehmann, Patricia J. Sime, Richard T. Libby, Krystel R. Huxlin et al.
Investigative Opthalmology & Visual Science
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A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010-06-08;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009-10-07;183(9):5799-806.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
Authors: Struyf S, Proost P, Vandercappellen J
Eur. J. Immunol., 2009-03-01;39(3):843-57.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
Clin. Cancer Res., 2007-10-01;13(19):5719-28.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood, 2004-03-25;104(2):314-20.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ELISA Development, ICC
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This antibody paired well with MAB279 to build an ELISA to measure MCP-1 in human serum samples.
