Human CD24 Antibody

Detection of Human CD24 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of CD24 in Human Colon.

CD24 was detected in immersion fixed paraffin-embedded sections of human colon using Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) at 1 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of CD24 by Western Blot

CASQ2 induces phenotypic changes in breast cancer cells. (A) Relationship between the proliferation rate and overexpression of CASQ2 in breast cancer cell lines (mean ± SEM, n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001 using the multiple t‐test). (B) Migration and invasion rates of Hs578T cells (mean ± SEM, n = 3; **P < 0.01 using two‐tailed Student’s t‐test). Scale bar = 50 μm. (C) Three‐dimensional culture of Hs578T cells (mean ± SEM, n = 3; **P < 0.01 using two‐tailed Student’s t‐test). Scale bar = 100 μm. (D) Tumorsphere culture of Hs578T cells (mean ± SEM, n = 3; **P < 0.01 using two‐tailed Student’s t‐test). Scale bar = 100 μm. (E) Expression of CD44, CD24, and ALDH1 cancer stem cell markers in adherent and tumorsphere cultures of Hs578T cells. (F) Measurement of intracellular Ca2+ in Hs578T cells using a calcium crimson reagent. Cells were loaded with the calcium indicator calcium crimson (5 μm) for 30 min, and then, BAPTA‐AM (10 μm) was added. After washing, the cells were stimulated by treatment with 5 μm caffeine to measure the intracellular calcium concentration at 360 s (mean ± SEM, n = 3; *P < 0.05 and **P < 0.01 after ANOVA with Tukey’s post hoc test). (G) Effect of lacidipine on the expression of cancer stem cell markers in tumorspheres of breast cancer cells. The figure shows one of three independent experiments. Statistical test result is shown in Fig. S6. (H) Epithelial–mesenchymal transition (EMT)‐related protein expression in breast cancer cells. All results are representatives of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34743414), licensed under a CC-BY license. Not internally tested by R&D Systems.