Human CD24 Biotinylated Antibody

Catalog #: FAB5247B Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5247B-100

Detection of CD24 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD24 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD24

Source

Monoclonal Mouse IgG_2A Clone # ML5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CD24

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD24 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P) and either (A) Mouse Anti-Human CD24 Biotinylated Monoclonal Antibody (Catalog # FAB5247B) or (B) Mouse IgG2A Biotinylated Isotype Control Antibody (IC003B) followed by APC-conjugated Streptavidin (F0050). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD24

CD24, also known as heat stable antigen (HSA), is a heavily and variably glycosylated 35‑60 kDa GPI-linked sialoprotein that is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is up‑regulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature human CD24 shares 30% and 42% aa sequence identity with mouse and rat CD24, respectively.

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Alternate Names

CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen