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Human CD25/IL-2R alpha Antibody

Catalog #: MAB223
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB223-SP
MAB223-100
MAB223-500
CD25/IL‑2 R alpha in Human PBMCs.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 4% cross-reactivity with recombinant human (rh) IL-2 R beta is observed and no cross-reactivity with rhIL-2 R gamma, rhIL-1 RI, rhIL-1 RII, rhIL-4 R, rhIL-5 R alpha, rhIL-6 R, rhIL‑7 R, rhIL-9 R, and rhIL-10 R is observed.
Source
Monoclonal Mouse IgG1 Clone # 22722
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD25/IL‑2 R alpha (Catalog # 223-2A) under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑2-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND50) is typically 0.5-1.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD25/IL-2 Ra antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD25/IL‑2 R alpha in Human PBMCs. CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. View Larger

Cell Proliferation Induced by IL‑2 and Neutralization by Human CD25/IL‑2 R alpha Antibody. Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223). The ND50 is typically 0.5-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name
Interleukin 2 Receptor alpha
Entrez Gene IDs
3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names
CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

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Citations for Human CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Alpha-synuclein fine-tunes neuronal response to pro-inflammatory cytokines
    Authors: Sigutova, V;Xiang, W;Regensburger, M;Winner, B;Prots, I;
    Brain, behavior, and immunity
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases
    Authors: Wu, WC;Shiu, C;Tong, TK;Leung, SO;Hui, CW;
    Journal of immunology research
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Identification of a CD4+ T cell line with Treg-like activity
    Authors: Thai H. Ho, Kirsten Pfeffer, Glen J. Weiss, Yvette Ruiz, Douglas F. Lake
    Human Immunology
  5. Restoring regulatory T-cell dysfunction in Alzheimer’s disease through ex vivo expansion
    Authors: Alireza Faridar, Aaron D Thome, Weihua Zhao, Jason R Thonhoff, David R Beers, Belen Pascual et al.
    Brain Communications
  6. Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK Cells
    Authors: F Porichis, MG Hart, A Massa, HL Everett, A Morou, J Richard, N Brassard, M Veillette, M Hassan, NL Ly, JP Routy, GJ Freeman, M Dubé, A Finzi, DE Kaufmann
    J. Immunol., 2018-06-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
    Sci Rep, 2016-09-02;6(0):32320.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  8. p120-catenin and beta -catenin differentially regulate cadherin adhesive function
    Authors: Rebecca G. Oas, Benjamin A. Nanes, Chimdimnma C. Esimai, Peter A. Vincent, Andrés J. García, Andrew P. Kowalczyk
    Molecular Biology of the Cell
  9. Cytokine modulation of defensive rage behavior in the cat: role of GABAA and interleukin-2 receptors in the medial hypothalamus.
    Authors: Bhatt S, Zalcman S, Hassanain M, Siegel A
    Neuroscience, 2005-01-01;133(1):17-28.
    Species: Feline
    Sample Types: In Vivo
    Applications: Neutralization

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Human CD25/IL-2R alpha Antibody
By Anonymous on 08/03/2020
Application: B/N Sample Tested: Recombinant protein Species: Human
Block/Neutralize Human CD25/IL-2R alpha Antibody MAB223