Human CD30/TNFRSF8 DuoSet ELISA

Name: Human CD30/TNFRSF8 DuoSet ELISA
Brand: R&D Systems
SKU: DY6126-05
Rating: 5 (1 reviews)

Catalog #: DY6126-05 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY6126-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

Human CD30 / TNFRSF8 ELISA Standard Curve

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All CD30/TNFRSF8 ELISAs

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CD30/TNFRSF8 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

93.8 - 6,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human CD30 / TNFRSF8 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD30/TNFRSF8

CD30/TNFRSF8 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The ligand for CD30 is CD30 Ligand/TNFSF8 (CD153), a member of the TNF superfamily. CD30 ligation by CD30 Ligand mediates pleiotropic effects, including cell proliferation, activation, differentiation and apoptosis.

Entrez Gene IDs:

943 (Human); 21941 (Mouse); 25069 (Rat)

Alternate Names:

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8