Human CD34 Antibody

Catalog #: MAB72274 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB72274-100
MAB72274-SP

Detection of CD34 in Human PBMCs by Flow Cytometry.

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Human CD34 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD34 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1054015

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human CD34 protein
Ser32-Thr290
Accession # P28906-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMCs and KG‑1 human acute myelogenous leukemia cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed KG-1 (positive) and K562 (negative) cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by APC-conjugated Anti-Mouse IgG secondary antibody and Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (FAB1430P). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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Detection of CD34 in KG-1 (positive) and K562 (negative) cells. CD34 was detected in immersion fixed KG-1 (positive) and absent in K562 (negative) cells using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry

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Detection of CD34 in KG-1 cells by Flow Cytometry. KG-1 cells were stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

References