Human CD34 Antibody

Catalog # Availability Size / Price Qty
MAB72274-100
MAB72274-SP
Detection of CD34 in Human PBMCs by Flow Cytometry.
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Human CD34 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISA.
Source
Monoclonal Mouse IgG2B Clone # 1054015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human CD34 protein
Ser32-Thr290
Accession # P28906-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human PBMCs and KG‑1 human acute myelogenous leukemia cell line
Immunocytochemistry
8-25 µg/mL
Immersion fixed KG-1 (positive) and K562 (negative) cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) or (B) Mouse IgG2B isotype control antibody (MAB0041) followed by APC-conjugated Anti-Mouse IgG secondary antibody and Mouse Anti-Human CD45 PE-conjugated Monoclonal Antibody (FAB1430P). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry View Larger

Detection of CD34 in KG-1 (positive) and K562 (negative) cells. CD34 was detected in immersion fixed KG-1 (positive) and absent in K562 (negative) cells using Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry View Larger

Detection of CD34 in KG-1 cells by Flow Cytometry. KG-1 cells were stained with Mouse Anti-Human CD34 Monoclonal Antibody (Catalog # MAB72274, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

References
  1. Civin C.I. et al. (1984) J. Immunol. 133:157.
  2. Katz F. et al. (1985) Leuk. Res. 9:191.
  3. Andrews R.G. et al. (1986) Blood 67:842.
  4. Young P.E. et al. (1995) Blood 85:96.
  5. Krause D.S. et al. (1996) Blood 87:1.
  6. Sidney L.E. et al. (2014) Stem Cells 32:1380.
Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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