Human CD34 Antibody Summary
Ser32-Thr290
Accession # P28906
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD34 in PBMC lymphocytes cells by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Human CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB1430P) and either (A) Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
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Detection of CD34 in KG‑1 cells (positive) and K562 (negative) cells. CD34 was detected in immersion fixed KG‑1 human acute myelogenous leukemia cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of CD34 in KG-1a cells by Flow Cytometry. KG-1a cells were stained with Rabbit Anti-Human CD34 Monoclonal Antibody (Catalog # MAB11441, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
- Civin C.I. et al. (1984) J. Immunol. 133:157.
- Katz F. et al. (1985) Leuk. Res. 9:191.
- Andrews R.G. et al. (1986) Blood 67:842.
- Young P.E. et al. (1995) Blood 85:96.
- Krause D.S. et al. (1996) Blood 87:1.
- Sidney L.E. et al. (2014) Stem Cells 32:1380.
Product Datasheets
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