Human CD34 Biotinylated Antibody

Catalog #: FAB7227B Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB7227B-100

Detection of CD34 in Human PBMCs by Flow Cytometry.

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Human CD34 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD34 in direct ELISAs

Source

Monoclonal Mouse IgG₁ Clone # QBEnd10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human endothelial vesicles

Formulation

PBS.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human CD34 Biotinylated Monoclonal Antibody (Catalog # FAB7227B) or (B) Biotinylated Mouse IgG1 isotype control antibody (IC002B) followed by PE-conjugated Streptavidin (F0040) and Mouse Anti-Human CD45 APC-conjugated Monoclonal Antibody (FAB1430A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1