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Human CD34 PE-conjugated Antibody

Catalog #: FAB7227P
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7227P
Detection of CD34 in Human PBMCs by Flow Cytometry.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD34 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD34 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # QBEnd10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human endothelial vesicles
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD34 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD34 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P) and Mouse Anti-Human CD45 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1430N). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD34 antibody in KG‑1a Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in KG‑1a Human Cell Line by Flow Cytometry. KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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Citations for Human CD34 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
    Authors: Iris A. Otto, Paulina Nuñez Bernal, Margot Rikkers, Mattie H.P. van Rijen, Anneloes Mensinga, Moshe Kon et al.
    iScience
  2. Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro*
    Authors: Letícia Martins SANTOS, Patricia e Silva CARDOSO, Elisa Abreu DINIZ, Juliana Garuba RAHHAL, Carla Renata SIPERT
    Journal of Applied Oral Science
  3. Exploration of T cell immune responses by expression of a dominant-negative SHP1 and SHP2
    Authors: Julia Taylor, Anna Bulek, Isaac Gannon, Mathew Robson, Evangelia Kokalaki, Thomas Grothier et al.
    Frontiers in Immunology
  4. Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients
    Authors: Francisco Raúl RA贚 BORZONE, María Belén BEL蒒 GIORELLO, Leandro Marcelo MARCELO MARTINEZ, María Cecilia CECILIA SANMARTIN, LEONARDO FELDMAN, FEDERICO DIMASE et al.
    Oncology Research
  5. Association of endothelial progenitor cells and peptic ulcer treatment in patients with type 2 diabetes mellitus
    Authors: ZHIHONG NIE, LIMIN XU, CHUANYUAN LI, TAO TIAN, PINGPING XIE, XIA CHEN et al.
    Experimental and Therapeutic Medicine
  6. Engineering second-generation TCR-T cells by site-specific integration of TRAF-binding motifs into the CD247 locus
    Authors: Lah S, Kim S, Kang I et al.
    Journal for immunotherapy of cancer
  7. Development of a TGFbeta-IL-2/15 Switch Receptor for Use in Adoptive Cell Therapy
    Authors: C Beck, NP Casey, I Persiconi, NN Moharrami, A Sike, Y Jin, JA Kyte
    Biomedicines, 2023-02-04;11(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Optimal Intravenous Administration Procedure for Efficient Delivery of Canine Adipose-Derived Mesenchymal Stem Cells
    Authors: Y Yasumura, T Teshima, Y Taira, T Saito, Y Yuchi, R Suzuki, H Matsumoto
    International Journal of Molecular Sciences, 2022-11-24;23(23):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Safe and effective off-the-shelf immunotherapy based on CAR.CD123-NK cells for the treatment of acute myeloid leukaemia
    Authors: S Caruso, B De Angelis, F Del Bufalo, R Ciccone, S Donsante, G Volpe, S Manni, M Guercio, M Pezzella, L Iaffaldano, DA Silvestris, M Sinibaldi, S Di Cecca, A Pitisci, E Velardi, P Merli, M Algeri, M Lodi, V Paganelli, M Serafini, M Riminucci, F Locatelli, C Quintarell
    Journal of hematology & oncology, 2022-11-05;15(1):163.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Human Wharton's Jelly Mesenchymal Stem Cells Secretome Inhibits Human SARS-CoV-2 and Avian Infectious Bronchitis Coronaviruses
    Authors: MAA Hussein, HAM Hussein, AA Thabet, KM Selim, MA Dawood, AM El-Adly, AA Wardany, A Sobhy, S Magdeldin, A Osama, AM Anwar, M Abdel-Waha, H Askar, EK Bakhiet, S Sultan, AA Ezzat, U Abdel Raou, MM Afifi
    Cells, 2022-04-21;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells
    Authors: T Teshima, K Okamoto, K Dairaku, T Nagashima, M Michishita, R Suzuki, H Matsumoto, H Koyama
    Stem Cells Int, 2020-10-18;2020(0):8841865.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Human beige adipocytes for drug discovery and cell therapy in metabolic diseases
    Authors: AM Singh, L Zhang, J Avery, A Yin, Y Du, H Wang, Z Li, H Fu, H Yin, S Dalton
    Nat Commun, 2020-06-02;11(1):2758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
    Authors: Y Xu, W Qin, D Guo, J Liu, M Zhang, Z Jin
    Biomed Res Int, 2019-06-23;2019(0):8735952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Clinical use of Dieletrophoresis separation for live Adipose derived stem cells.
    J Transl Med, 2012-05-17;10(0):99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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