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Human CD45 APC-conjugated Antibody

Catalog #: FAB1430A
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1430A-100
FAB1430A-025
Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (7)
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Human CD45 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD45. This antibody recognizes all isoforms of human CD45.
Source
Monoclonal Mouse IgG1 Clone # 2D1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human peripheral blood mononuclear cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD45 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB1430A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD45

CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the class 1 (receptor‑like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The CD45RABC cDNA encodes 1304 amino acids (aa), including a 23 aa signal sequence, a 552 aa extracellular domain containing the splicing region, a cysteine‑rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 707 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode aa 32‑191. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine‑tuned by varying usage of the heavily O‑glycosylated spliced regions and sialylation of N‑linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the Src family kinase, Lck (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP‑1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

References
  1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
  2. Saunders, E.A. and P. Johnson. (2010) Cell Signal. 22:339.
  3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  4. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608.
  5. Ralph, S.J. et al. (1987) EMBO J. 6:1251.
  6. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082.
  7. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146.
  8. McNiell, L. et al. (2007) Immunity 27:425.
  9. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361.
  10. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911.
  11. Zhu, J.W. et al. (2008) Immunity 28:183.
  12. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190.
  13. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012.
  14. Cross, J.L. et al. (2008) J. Immunol. 180:8020.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names
B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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Citations for Human CD45 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Effect of monocyte seeding density on dendritic cell generation in an automated perfusion-based culture system
    Authors: Andrew Kozbial, Lekhana Bhandary, Shashi K. Murthy
    Biochemical Engineering Journal
  2. LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression
    Authors: Y Xu, W Qin, D Guo, J Liu, M Zhang, Z Jin
    Biomed Res Int, 2019-06-23;2019(0):8735952.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients
    Authors: Y Tang, Z Wang, Z Li, J Kim, Y Deng, Y Li, JR Heath, W Wei, S Lu, Q Shi
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The differentiation potential of gingival mesenchymal stem cells induced by apical tooth germ cell?conditioned medium
    Mol Med Rep, 2016-09-06;14(4):3565-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Choice of xenogenic-free expansion media significantly influences the myogenic differentiation potential of human bone marrow-derived mesenchymal stromal cells.
    Authors: Brun J, Abruzzese T, Rolauffs B, Aicher W, Hart M
    Cytotherapy, 2016-03-01;18(3):344-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-alpha monotherapy.
    Authors: Ilander M, Kreutzman A, Rohon P, Melo T, Faber E, Porkka K, Vakkila J, Mustjoki S
    PLoS ONE, 2014-01-31;9(1):e87794.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
    Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
    Cytometry A, 2011-07-06;79(8):635-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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