Human CD45RA Antibody

Catalog #: FAB11444MFV450 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB11444MFV450-100

Detection of CD45RA in PBMC lymphocytes by Flow Cytometry

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Human CD45RA Antibody Summary

Species Reactivity

Human

Specificity

Detects CD45RA in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1069433

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Whole cells (naïve T cells)
Accession # P08575

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet(SDS) for additional information and handling instructions.

Label

mFluor Violet 450 SE (Excitation= 406 nm, Emission= 445 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD45RA in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P) and either (A) Mouse Anti-Human CD45RA mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB11444MFV450) or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: CD45RA

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA. CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene. In humans, CD45RA is one of six isoforms of the CD45 complex. These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC. CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells. CD45RA contains exon 4 but lacks both exons 5 and 6. CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons. Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells. Other cell types such as activated monocytes and B cells can also express CD45RO. Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Long Name

CD45R, A isoform

Entrez Gene IDs

5788 (Human)

Alternate Names

B220; CD45 antigen; CD45R; CD45RA; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide