Human CD63 Antibody Summary
Ala103-Val203
Accession # P08962-1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD63 by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD63 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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CD63 in Human Lung Cancer Tissue. CD63 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Detection of CD63 in Human PBMC by Flow Cytometry. Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
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Detection of Human CD63 by Simple WesternTM. Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of CD63 in PBMC monocytes by Flow Cytometry PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD63
CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of human CD63 share 67% and 65% amino acid sequence identity with mouse and rat CD63, respectively.
Product Datasheets
Citations for Human CD63 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Exosomal miR-105-5p derived from bladder cancer stem cells targets for GPR12 to promote the malignancy of bladder cancer
Authors: Pan, G;Jiang, B;Yi, Z;Yin, J;Liu, Y;
BMC urology
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Disrupting the alpha-synuclein-ESCRT interaction with a peptide inhibitor mitigates neurodegeneration in preclinical models of Parkinson's disease
Authors: S Nim, DM O'Hara, C Corbi-Verg, A Perez-Riba, K Fujisawa, M Kapadia, H Chau, F Albanese, G Pawar, ML De Snoo, SG Ngana, J Kim, OMA El-Agnaf, E Rennella, LE Kay, SK Kalia, LV Kalia, PM Kim
Nature Communications, 2023-04-19;14(1):2150.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular vesicle-derived long non-coding RNA as circulating biomarkers for endometriosis
Authors: S Shan, Y Yang, J Jiang, B Yang, Y Yang, F Sun, J Zhang, Y Lin, H Xu
Reproductive biomedicine online, 2021-12-01;0(0):.
Species: Human
Sample Types: Extracellular Vesicles
Applications: Cell Isolation, SDS-Page, Western Blot
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