Human CD74 PerCP-conjugated Antibody

Name: Human CD74 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB35901C

Catalog #: FAB35901C Datasheet / COA / SDS

Discontinued Product

FAB35901C has been discontinued.

View all CD74 products.

Detection of CD74 in Human Blood Lymphocytes by Flow Cytometry.

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All CD74 Antibodies

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Human CD74 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human CD74 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 332516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-S-derived recombinant human CD74
Gln73-Met232
Accession # NP_004346

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD74 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD74 PerCP-conjugated Monoclonal Antibody (Catalog # FAB35901C) or (B) Mouse IgG_2BPerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD74

CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappa B. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.

Entrez Gene IDs

972 (Human); 16149 (Mouse); 25599 (Rat)

Alternate Names

CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33