Human CD96v2 Antibody

Catalog #: MAB6199
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6199
MAB6199-SP

Detection of CD96 in Human PBMCs by Flow Cytometry.

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All CD96 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD96v2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD96 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 628211

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD96
Val22-Asp501
Accession # NP_005807

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD96 in Human PBMCs by Flow Cytometry. Human peripheral blood cells were stained with Human CD96 Monoclonal Antibody (Catalog # MAB6199) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4⁺ and CD8⁺ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155⁺ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile

Product Datasheets

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Related Research Areas

Citation for Human CD96v2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Fc-gamma receptor-mediated crosslinking co-defines the immunostimulatory activity of anti-human CD96 antibodies
Authors: A Rogel, FM Ibrahim, SM Thirdborou, F Renart-Dep, CN Birts, SL Buchan, X Preville, EV King, A Al-Shamkha
JCI Insight, 2022-10-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry