Human CEACAM-18 APC-conjugated Antibody

Catalog #: FAB9869A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB9869A-100

Detection of CEACAM-18 in HEK293 cells by Flow Cytometry.

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All CEACAM-18 Antibodies

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Human CEACAM-18 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-18 in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1057633

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human CEACAM-18
Gln31-His317
Accession # A8MTB9

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

HEK293 cells transfected with Human CEACAM-18 vs Irrelevant transfectant cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM-18 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human CEACAM-18 (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human CEACAM-18 APC‑conjugated Monoclonal Antibody (Catalog # FAB9869A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-18

Carcinoembryonic Antigen-related Cell Adhesion Molecule 18 (CEACAM-18) is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-18 has a 298 amino acid (aa) extracellular domain containing 2 IgC2-like and 1 IgV-like domains, a single transmembrane domain and a short cytoplasmic tail (2). CEACAM-18 is one of only five conserved CEACAMs among mouse, rat, and human (2), but mature human CEACAM-18 has low aa sequence identify with mouse and rat at 60% and 58%, respectively. Originally discovered as a biomarker for colorectal cancer (3), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (4-6). While the exact function of CEACAM-18 has been yet to be elucidated, it may bind pathogen receptors or other immunoregulatory members (6). CEACAM family members were identified as the major Galectin-3 receptor candidates on human neutrophils (7). Binding of carbohydrate ligands to CEACAMs may be important in the release of proinflammatory mediators (8, 9).

References