Human CEACAM-8/CD66b APC-conjugated Antibody

Name: Human CEACAM-8/CD66b APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB4246A
Price: 151 USD

Catalog #: FAB4246A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Discontinued Product

FAB4246A has been discontinued.

View all CEACAM-8/CD66b products.

Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry.

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All CEACAM-8/CD66b Antibodies

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Human CEACAM-8/CD66b APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human CEACAM‑8/CD66b transfectants but not irrelevant transfectants in flow cytometry.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 913542

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM-8/CD66b APC-conjugated Monoclonal Antibody (Catalog # FAB4246A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CEACAM‑8/CD66b in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human CEACAM-8/CD66b or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM-8/CD66b APC-conjugated Monoclonal Antibody (Catalog # FAB4246A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-8/CD66b

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8) also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Entrez Gene IDs

1088 (Human); 102138843 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95