Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit Summary
Sample Values
Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human CHI3L1 in this assay. No medical histories were available for the donors used in this study.Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
Serum (n=36) | 36.0 | 15.9-93.5 | 20.6 |
EDTA plasma (n=36) | 27.7 | 10.9-79.1 | 17.5 |
Heparin plasma (n=36) | 26.8 | 10.7-72.1 | 15.7 |
Urine (n=11) | 0.64 | 0.14-1.82 | 0.49 |
Cell Culture Supernates:
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 566 | 1031 | 2202 | 606 | 1019 | 2212 |
Standard Deviation | 26.7 | 44.4 | 102.6 | 32.1 | 59.1 | 153.1 |
CV% | 4.7 | 4.3 | 4.7 | 5.3 | 5.8 | 6.9 |
Recovery
The recovery of CHI3L1 spiked to levels throughout the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 97 | 93-100 |
Urine (n=4) | 105 | 90-115 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Chitinase 3-like 1/YKL-40
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Chitinase 3-like 1/YKL-40 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Early Fever in Allogeneic Stem Cell Transplantation: Are Presepsin and YKL-40 Valuable Diagnostic Tools?
Authors: Babel, J;Kouta, I;Vujaklija Brajkovi?, A;Lon?ar Vran?i?, A;Premui?, V;Rogi?, D;Durakovi?, N;
Journal of clinical medicine
Species: Human
Sample Types: Serum
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Alzheimer's disease CSF biomarkers correlate with early pathology and alterations in neuronal and glial gene expression
Authors: Ropri, AS;Lam, TG;Kalia, V;Buchanan, HM;Bartosch, AMW;Youth, EHH;Xiao, H;Ross, SK;Jain, A;Chakrabarty, JK;Kang, MS;Boyett, D;Spinazzi, EF;Iodice, G;McGovern, RA;Honig, LS;Brown, LM;Miller, GW;McKhann, GM;Teich, AF;
medRxiv : the preprint server for health sciences
Species: Human
Sample Types: CSF
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p75 neurotrophin receptor modulation in mild to moderate Alzheimer disease: a randomized, placebo-controlled phase 2a trial
Authors: Shanks, HRC;Chen, K;Reiman, EM;Blennow, K;Cummings, JL;Massa, SM;Longo, FM;Börjesson-Hanson, A;Windisch, M;Schmitz, TW;
Nature medicine
Species: Human
Sample Types: CSF
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Heavy Metals and Essential Metals Are Associated with Cerebrospinal Fluid Biomarkers of Alzheimer's Disease
Authors: M Babi? Leko, M Mihel?i?, J Jurasovi?, M Nikolac Pe, E Špani?, A Sekovani?, T Orct, K Zub?i?, L Langer Hor, N Plei?, S Ki?emet-Pi, Ž Vogrinc, N Pivac, A Diana, F Borove?ki, PR Hof, G Šimi?
International Journal of Molecular Sciences, 2022-12-27;24(1):.
Species: Human
Sample Types: CSF
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Plasma Human Cartilage Glycoprotein-39 Is Associated With the Prognosis of Acute Ischemic Stroke
Authors: T Xu, K Zhang, C Zhong, Z Zhu, X Zheng, P Yang, B Che, Y Lu, Y Zhang
Journal of the American Heart Association, 2022-09-14;0(0):e026263.
Species: Human
Sample Types: Serum
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Association of Serum GFAP with Functional and Neurocognitive Outcome in Sporadic Small Vessel Disease
Authors: A Huss, A Abdelhak, B Mayer, H Tumani, HP Müller, K Althaus, J Kassubek, M Otto, AC Ludolph, D Yilmazer-H, H Neugebauer
Biomedicines, 2022-08-02;10(8):.
Species: Human
Sample Types: CSF
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Human iPSC co-culture model to investigate the interaction between microglia and motor neurons
Authors: BF Vahsen, E Gray, A Candalija, KML Cramb, J Scaber, R Dafinca, A Katsikoudi, Y Xu, L Farrimond, R Wade-Marti, WS James, MR Turner, SA Cowley, K Talbot
Scientific Reports, 2022-07-23;12(1):12606.
Species: Human
Sample Types: Cell Culture Supernates
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Serological profiling reveals hsa-miR-451a as a possible biomarker of anaphylaxis
Authors: W Francuzik, K Pažur, M Dalke, S Dölle-Bier, M Babina, M Worm
JCI Insight, 2022-04-08;0(0):.
Species: Human
Sample Types: Serum
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Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
Regenerative Therapy, 2021-03-27;18(0):38-50.
Species: Human
Sample Types: Cell Culture Supernates
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Increased plasma levels of chitinase 3-like 1 (CHI3L1) protein in patients with idiopathic normal-pressure hydrocephalus
Authors: PW Ko, HW Lee, M Lee, YC Youn, S Kim, JH Kim, K Kang, K Suk
Journal of the neurological sciences, 2021-02-20;423(0):117353.
Species: Human
Sample Types: Plasma
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The potential pathways underlying the association of propyl-paraben exposure with aeroallergen sensitization and EASI score using metabolomics analysis
Authors: Y Lee, E Lee, DK Yon, HM Jee, HS Baek, SW Lee, JY Cho, MY Han
Scientific Reports, 2021-02-12;11(1):3772.
Species: Human
Sample Types: Serum
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Cerebrospinal Fluid Biomarkers in Relation to MRZ Reaction Status in Primary Progressive Multiple Sclerosis
Authors: T Robinson, A Abdelhak, T Bose, E Meinl, M Otto, UK Zettl, R Dersch, H Tumani, S Rauer, A Huss
Cells, 2020-11-25;9(12):.
Species: Human
Sample Types: CSF
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Serum YKL-40 Levels Are Associated with the Atherogenic Index of Plasma in Children
Authors: Y Kwon, JH Kim, EK Ha, HM Jee, HS Baek, MY Han, SJ Jeong
Mediators Inflamm, 2020-09-26;2020(0):8713908.
Species: Human
Sample Types: Serum
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A Multi-Omics Approach Reveals New Signatures in Obese Allergic Asthmatic Children
Authors: MA Gomez-Llor, A Martínez-C, N Chueca, MC Rico, R Romero, A Anguita-Ru, CM Aguilera, M Gil-Campos, MD Mesa, B Khakimov, JA Morillo, Á Gil, J Camacho, C Gomez-Llor
Biomedicines, 2020-09-18;8(9):.
Species: Human
Sample Types: Plasma
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Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis
Authors: G Ye, Z Xie, H Zeng, P Wang, J Li, G Zheng, S Wang, Q Cao, M Li, W Liu, S Cen, Z Li, Y Wu, Z Ye, H Shen
Cell Death Dis, 2020-09-17;11(9):775.
Species: Human
Sample Types: Serum
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Association of glial and neuronal degeneration markers with Alzheimer's disease cerebrospinal fluid profile and cognitive functions
Authors: UD Teitsdotti, MK Jonsdottir, SH Lund, T Darreh-Sho, J Snaedal, PH Petersen
Alzheimers Res Ther, 2020-08-04;12(1):92.
Species: Human
Sample Types: CSF
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Combined assessment of serum eosinophil-derived neurotoxin and YKL-40 may identify Asthma-COPD overlap
Authors: T Shirai, K Hirai, Y Gon, S Maruoka, K Mizumura, M Hikichi, K Itoh, S Hashimoto
Allergol Int, 2020-06-20;0(0):.
Species: Human
Sample Types: Serum
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Determination of chitinase 3-like 1 in cerebrospinal fluid in multiple sclerosis and other neurological diseases
Authors: P Kušnierová, D Zeman, P Hradílek, O Zapletalov, D Stejskal
PLoS ONE, 2020-05-21;15(5):e0233519.
Species: Human
Sample Types: CSF
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Urinary cell cycle arrest biomarkers and chitinase 3-like protein 1 (CHI3L1) to detect acute kidney injury in the critically ill: a post hoc laboratory analysis on the FINNAKI cohort
Authors: EA Hoste, ST Vaara, J De Loor, M Haapio, L Nuytinck, K Demeyere, V Pettilä, E Meyer
Crit Care, 2020-04-10;24(1):144.
Species: Human
Sample Types: Urine
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Improved early detection of ovarian cancer using longitudinal multimarker models
Authors: HJ Whitwell, J Worthingto, O Blyuss, A Gentry-Mah, A Ryan, R Gunu, J Kalsi, U Menon, I Jacobs, A Zaikin, JF Timms
Br. J. Cancer, 2020-01-15;0(0):.
Species: Human
Sample Types: Serum
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Cerebrospinal fluid and plasma biomarker trajectories with increasing amyloid deposition in Alzheimer's disease
Authors: S Palmqvist, PS Insel, E Stomrud, S Janelidze, H Zetterberg, B Brix, U Eichenlaub, JL Dage, X Chai, K Blennow, N Mattsson, O Hansson
EMBO Mol Med, 2019-11-11;0(0):e11170.
Species: Human
Sample Types: CSF
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Cell-free mitochondrial DNA in progressive multiple sclerosis
Authors: H Lowes, A Pyle, M Duddy, G Hudson
Mitochondrion, 2018-08-08;0(0):.
Species: Human
Sample Types: CSF
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Sleep Deprivation and CSF Biomarkers for Alzheimer Disease
Authors: M Olsson, J Ärlig, J Hedner, K Blennow, H Zetterberg
Sleep, 2018-05-01;0(0):.
Species: Human
Sample Types: CSF
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Effects of surgery and propofol-remifentanil total intravenous anesthesia on cerebrospinal fluid biomarkers of inflammation, Alzheimer's disease, and neuronal injury in humans: a cohort study
Authors: A Pikwer, M Castegren, S Namdar, K Blennow, H Zetterberg, N Mattsson
J Neuroinflammation, 2017-09-29;14(1):193.
Species: Human
Sample Types: CSF
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Chitinase-3-Like Protein 1 (YKL-40) Is a New Biomarker of Inflammation in Psoriasis
Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
Mediators Inflamm., 2017-08-28;2017(0):9538451.
Species: Human
Sample Types: Serum
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Chitinase-3-Like Protein 1 (YKL-40) Reflects the Severity of Symptoms in Atopic Dermatitis
Authors: J Salomon, ? Matusiak, D Nowicka-Su, JC Szepietows
J Immunol Res, 2017-06-04;2017(0):5746031.
Species: Human
Sample Types: Serum
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Effect of physical exercise on markers of neuronal dysfunction in cerebrospinal fluid in patients with Alzheimer's disease
Authors: CS Jensen, E Portelius, P Høgh, L Wermuth, K Blennow, H Zetterberg, SG Hasselbalc, AH Simonsen
Alzheimers Dement (N Y), 2017-04-17;3(2):284-290.
Species: Human
Sample Types: CSF
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Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017-03-20;0(0):.
Species: Human
Sample Types: Plasma
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Diagnosis of cardiac surgery-associated acute kidney injury: differential roles of creatinine, chitinase 3-like protein 1 and neutrophil gelatinase-associated lipocalin: a prospective cohort study
Authors: J De Loor, I Herck, K Francois, A Van Wesema, L Nuytinck, E Meyer, EA Hoste
Ann Intensive Care, 2017-03-01;7(1):24.
Species: Human
Sample Types: Urine
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Cerebrospinal Fluid Biomarkers of Neurodegeneration Are Decreased or Normal in Narcolepsy
Authors: P Jørgen Jen, L Østergaard, JM Czarna Bah, S Modvig, K Fog, A Holm, B Rahbek Kor, S Gammeltoft
Sleep, 2017-01-01;40(1):.
Species: Human
Sample Types: CSF
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Cerebrospinal fluid microglia and neurodegenerative markers in twins concordant and discordant for psychotic disorders
Authors: Viktoria Johansson
Eur Arch Psychiatry Clin Neurosci, 2016-12-30;0(0):.
Species: Human
Sample Types: CSF
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Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
JCI Insight, 2016-12-08;1(20):e90954.
Species: Human
Sample Types: Cell Culture Supernates
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Least absolute shrinkage and selection operator type methods for the identification of serum biomarkers of overweight and obesity: simulation and application
BMC Med Res Methodol, 2016-11-14;16(1):154.
Species: Human
Sample Types: Serum
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MiR-205 and MiR-373 Are Associated with Aggressive Human Mucinous Colorectal Cancer
Authors: Annette Eyking
PLoS ONE, 2016-06-06;11(6):e0156871.
Species: Human
Sample Types: Cell Culture Supernates
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Airway, but not serum or urinary, levels of YKL-40 reflect inflammation in early cystic fibrosis lung disease.
Authors: Fantino E, Gangell C, Hartl D, Sly P
BMC Pulm Med, 2014-02-27;14(0):28.
Species: Human
Sample Types: BALF
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Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
PLoS ONE, 2013-04-22;8(4):e61984.
Species: Human
Sample Types: Serum
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Levels of YKL-40 in pleural effusions and blood from patients with pulmonary or pleural disease.
Authors: Kim HR, Jun CD, Lee KS, Cho JH, Jeong ET, Yang SH, Lee YJ, Park DS
Cytokine, 2012-04-03;58(3):336-43.
Species: Human
Sample Types: Serum
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The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
Authors: Hector A, Kormann MS, Mack I, Latzin P, Casaulta C, Kieninger E, Zhou Z, Yildirim AÖ, Bohla A, Rieber N, Kappler M, Koller B, Eber E, Eickmeier O, Zielen S, Eickelberg O, Griese M, Mall MA, Hartl D
PLoS ONE, 2011-09-20;6(9):e24399.
Species: Human
Sample Types: Serum
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