Home / CLEC-1 / Human CLEC-1 Antibody

Human CLEC-1 Antibody

Catalog #: MAB1704
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1704
MAB1704-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CLEC-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 219003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-1
Gln77-Asp280
Accession # Q8NC01
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CLEC‑1 (Catalog # 1704-CL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-1

CLEC-1 (C-type lectin-like receptor-1) is a type 2 transmembrane protein that belongs to the Dectin-1 family of C-type lectins (1, 2). It consists of a 52 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 207 aa extracellular domain (ECD) (3). The ECD contains a carbohydrate recognition domain (CRD) that includes cysteine residues with conserved spacing (4). In other C-type lectins, these cysteines form intrachain disulfide bonds that stabilize the CRD protein fold (4). CLEC-1 is a nonclassical C-type lectin in that its CRD lacks consensus amino acids which mediate calcium dependent carbohydrate binding in other C-type lectins (2, 3). The cytoplasmic domain contains one tyrosine, multiple serine and threonine, and a cluster of aspartic acid residues. The CLEC-1, CLEC-2, Dectin-1, and LOX-1 genes are located within the NK gene complex that also encodes several receptors involved in regulating NK cell activation (3, 5). CLEC-1 is most highly expressed in activated dendritic cells and at lower levels in endothelial cells and monocytes (3, 5). Within the CRD, human CLEC‑1 shares 65% aa sequence identity with mouse and rat CLEC-1 and 26%‑36% with the other human Dectin-1 family lectins CLEC-2, CLEC9A, CLEC12B, Dectin-1, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Pyz, E. et al. (2006) Ann. Med. 38:242.
  3. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  4. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  5. Sobanov, Y. et al. (2001) Eur. J. Immunol. 31:3493.
Long Name
C-type Lectin-like Receptor-1
Entrez Gene IDs
51267 (Human); 243653 (Mouse)
Alternate Names
CLEC1; CLEC-1; CLEC1A; CLEC1C-type lectin domain family 1 member A; C-type lectin domain family 1, member A; C-type lectin-like receptor 1; C-type lectin-like receptor-1; MGC34328

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human CLEC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis
    Authors: Zhu G, Lyu L, Yang H et al.
    Investigative ophthalmology & visual science
  2. CLEC-1 is a death sensor that limits antigen cross-presentation by dendritic cells and represents a target for cancer immunotherapy
    Authors: M Drouin, J Saenz, V Gauttier, B Evrard, G Teppaz, S Pengam, C Mary, A Desselle, V Thepenier, E Wilhelm, E Merieau, C Ligeron, I Girault, MD Lopez, C Fourgeux, D Sinha, I Baccelli, A Moreau, C Louvet, R Josien, J Poschmann, N Poirier, E Chiffoleau
    Science Advances, 2022-11-18;8(46):eabo7621.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. Histidine-Rich Glycoprotein Stimulates Human Neutrophil Phagocytosis and Prolongs Survival through CLEC1A
    Authors: Yohei Takahashi, Hidenori Wake, Masakiyo Sakaguchi, Yukinori Yoshii, Kiyoshi Teshigawara, Dengli Wang et al.
    The Journal of Immunology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human CLEC-1 Antibody

There are currently no reviews for this product. Be the first to review Human CLEC-1 Antibody and earn rewards!

Have you used Human CLEC-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review