Human DCIR/CLEC4A Antibody

Name: Human DCIR/CLEC4A Antibody
Brand: R&D Systems
SKU: MAB1748
Rating: 4 (1 reviews)

Catalog #: MAB1748
3 Citations 1 Review Datasheet / COA / SDS

Clone 216110 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
MAB1748-SP
MAB1748-100
MAB1748-500

All DCIR/CLEC4A Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DCIR/CLEC4A Antibody Summary

Species Reactivity

Human

Specificity

Detects human DCIR in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 216110

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DCIR isoform 1
Gln70-Leu237
Accession # Q9UMR7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human DCIR/CLEC4A
under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood neutrophils

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DCIR/CLEC4A

DCIR, also known as Lectin-like Immunoreceptor (LLIR), is a type II membrane protein belonging to the C-type lectin domain family and is designated CLEC4A (previously designated CLECSF6). Four transcript variants encoding distinct isoforms have been identified. DCIR contains one carbohydrate recognition domain in its C-terminal extracellular domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. Besides DC, DCIR is expressed on B cells, monocytes/macrophages and granulocytes.

Long Name

Dendritic Cell Immunoreceptor

Entrez Gene IDs

50856 (Human); 26888 (Mouse)

Alternate Names

CD367; CLEC4A; CLECSF6; CLECSF6C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 6; C-type lectin domain family 4 member A; C-type lectin domain family 4, member A; DCIR; DCIRLLIR; DDB27; DDB27C-type lectin DDB27; Dendritic cell immunoreceptor; Lectin-like immunoreceptor; LLIR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human DCIR/CLEC4A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2010-10-07;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
DCIR is endocytosed into human dendritic cells and inhibits TLR8-mediated cytokine production.
Authors: Meyer-Wentrup F, Cambi A, Joosten B, Looman MW, de Vries IJ, Figdor CG, Adema GJ
J. Leukoc. Biol., 2008-11-21;85(3):518-25.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Immunoprecipitation
Targeting DCIR on human plasmacytoid dendritic cells results in antigen presentation and inhibits IFN-alpha production.
Authors: Meyer-Wentrup F, Benitez-Ribas D, Tacken PJ, Punt CJ, Figdor CG, de Vries IJ, Adema GJ
Blood, 2008-02-07;111(0):4245.
Species: Human
Sample Types: Whole Cells
Applications: Cross-linking