Home / Dectin-1/CLEC7A / Human Dectin-1/CLEC7A Antibody

Human Dectin-1/CLEC7A Antibody

Catalog #: MAB1859
34 Citations 3 Reviews Datasheet / COA / SDS
Clone 259931 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1859
MAB1859-SP
Dectin‑1/CLEC7A in Human Dendritic Cells.
1 Image
Product Details
Citations (34)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 259931
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
Thr66-Met201
Accession # NP_072092
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Dectin-1/CLEC7A antibody in Human Dendritic Cells by Immunocytochemistry (ICC). View Larger

Dectin‑1/CLEC7A in Human Dendritic Cells. Dectin-1/CLEC7A was detected in immersion fixed mature human dendritic cells using Mouse Anti-Human Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB1859) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Glycosphingolipids Mediate Pneumocystis Cell Wall beta -Glucan Activation of the IL-23/IL-17 Axis in Human Dendritic Cells
    Authors: Eva M. Carmona, Theodore J. Kottom, Deanne M. Hebrink, Teng Moua, Raman-Deep Singh, Richard E. Pagano et al.
    American Journal of Respiratory Cell and Molecular Biology
  2. The C-Type Lectin Receptor DC-SIGN Has an Anti-Inflammatory Role in Human M(IL-4) Macrophages in Response to Mycobacterium tuberculosis
    Authors: Geanncarlo Lugo-Villarino, Anthony Troegeler, Luciana Balboa, Claire Lastrucci, Carine Duval, Ingrid Mercier et al.
    Frontiers in Immunology
  3. CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis
    Authors: Zhu G, Lyu L, Yang H et al.
    Investigative ophthalmology & visual science
  4. Genome-Wide Association Study Reveals CLEC7A and PROM1 as Potential Regulators of Paracoccidioides brasiliensis-Induction of Cytokine Production in Peripheral Blood Mononuclear Cells
    Authors: Ana Marina B. de Figueiredo, Jéssica Cristina dos Santos, Brenda Kischkel, Edwin Ardiansyah, Marije Oosting, Grazzielle Guimarães Matos et al.
    Journal of Fungi
  5. Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity
    Authors: Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Dectin-1-Independent Macrophage Phagocytosis of Mycobacterium abscessus
    Authors: Ochoa, AE;Congel, JH;Corley, JM;Janssen, WJ;Nick, JA;Malcolm, KC;Hisert, KB;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2
    Authors: S Haji, T Ito, C Guenther, M Nakano, T Shimizu, D Mori, Y Chiba, M Tanaka, SK Mishra, JA Willment, GD Brown, M Nagae, S Yamasaki
    Elife, 2022-12-08;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs
    Authors: SI Gringhuis, TM Kaptein, EBM Remmerswaa, A Drewniak, BA Wevers, B Theelen, GRAM D'Haens, T Boekhout, TBH Geijtenbee
    Nature Immunology, 2022-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Essential role of TOSO/FAIM3 in intestinal IgM reverse transcytosis
    Authors: N Rochereau, E Michaud, L Waeckel, M Killian, R Gayet, R Goguyer-De, X Roblin, G Biolley, B Corthésy, S Paul
    Cell Reports, 2021-11-16;37(7):110006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021-07-20;36(3):109392.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  11. Mac-1 triggers neutrophil DNA extracellular trap formation to Aspergillus fumigatus independently of PAD4 histone citrullination
    Authors: JC Silva, NC Rodrigues, GA Thompson-S, VS Muniz, JS Neves, RT Figueiredo
    J. Leukoc. Biol., 2019-09-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis
    Authors: JC de Quaglia, AM Della Cole, TP Gardizani, GG Romagnoli, R Kaneno, LA Dias-Melic
    J Immunol Res, 2019-02-06;2019(0):1529189.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Cellular metabolism constrains innate immune responses in early human ontogeny
    Authors: B Kan, C Michalski, H Fu, HHT Au, K Lee, EA Marchant, MF Cheng, E Anderson-B, M Aharoni-Si, P Tilley, RG Mirmira, CJ Ross, DS Luciani, E Jan, PM Lavoie
    Nat Commun, 2018-11-16;9(1):4822.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses
    Authors: MMM Kaisar, M Ritter, C Del Fresno, HS Jónasdótti, AJ van der Ha, LR Pelgrom, G Schramm, LE Layland, D Sancho, C Prazeres d, M Giera, M Yazdanbakh, B Everts
    PLoS Biol., 2018-04-18;16(4):e2005504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. EphA2 is an epithelial cell pattern recognition receptor for fungal ?-glucans
    Authors: M Swidergall, NV Solis, MS Lionakis, SG Filler
    Nat Microbiol, 2017-11-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  16. Dectin-1-Mediated Pathway Contributes to Fusarium proliferatum-Induced CXCL-8 Release from Human Respiratory Epithelial Cells
    Authors: CC Yeh, HC Horng, H Chou, HY Tai, HD Shen, SL Hsieh, PH Wang
    Int J Mol Sci, 2017-03-13;18(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
    PLoS ONE, 2016-11-29;11(11):e0167410.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Human Neutrophils Use Different Mechanisms To Kill Aspergillus fumigatus Conidia and Hyphae: Evidence from Phagocyte Defects.
    Authors: Gazendam R, van Hamme J, Tool A, Hoogenboezem M, van den Berg J, Prins J, Vitkov L, van de Veerdonk F, van den Berg T, Roos D, Kuijpers T
    J Immunol, 2015-12-30;196(3):1272-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. TSLP production by dendritic cells is modulated by IL-1beta and components of the endoplasmic reticulum stress response.
    Authors: Elder M, Webster S, Williams D, Gaston J, Goodall J
    Eur J Immunol, 2015-12-02;46(2):455-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Opposing Roles of Dectin-1 Expressed on Human Plasmacytoid Dendritic Cells and Myeloid Dendritic Cells in Th2 Polarization.
    Authors: Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li X, Hori T, Zurawski S, Liu Y, Zurawski G, Oh S
    J Immunol, 2015-06-29;195(4):1723-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
    Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
    PLoS ONE, 2015-03-20;10(3):e0120948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  22. Human intestinal epithelial cells respond to beta-glucans via Dectin-1 and Syk.
    Authors: Cohen-Kedar S, Baram L, Elad H, Brazowski E, Guzner-Gur H, Dotan I
    Eur J Immunol, 2014-10-27;44(12):3729-40.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  23. Interferon-gamma production by human neutrophils upon stimulation by IL-12, IL-15 and IL-18 and challenge with Paracoccidioides brasiliensis.
    Authors: Rodrigues D, Fernandes R, Balderramas H, Penitenti M, Bachiega T, Calvi S, Dias-Melicio L, Ikoma M, Soares A
    Cytokine, 2014-06-14;69(1):102-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Podosomes of dendritic cells facilitate antigen sampling.
    Authors: Baranov, Maksim V, Ter Beest, Martin, Reinieren-Beeren, Inge, Cambi, Alessand, Figdor, Carl G, van den Bogaart, Geert
    J Cell Sci, 2014-01-14;127(0):1052-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. The C-type lectin receptors dectin-1, MR, and SIGNR3 contribute both positively and negatively to the macrophage response to Leishmania infantum.
    Authors: Lefevre L, Lugo-Villarino G, Meunier E, Valentin A, Olagnier D, Authier H, Duval C, Dardenne C, Bernad J, Lemesre J, Auwerx J, Neyrolles O, Pipy B, Coste A
    Immunity, 2013-05-16;38(5):1038-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway.
    Authors: Ifrim D, Joosten L, Kullberg B, Jacobs L, Jansen T, Williams D, Gow N, van der Meer J, Netea M, Quintin J
    J Immunol, 2013-03-08;190(8):4129-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  27. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
    Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
    PLoS ONE, 2012-12-11;7(12):e50589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.
    Authors: Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ
    Eur. J. Immunol., 2012-06-01;42(6):1512-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  29. Dectin-1 is an extracellular pathogen sensor for the induction and processing of IL-1beta via a noncanonical caspase-8 inflammasome.
    Authors: Gringhuis SI, Kaptein TM, Wevers BA, Theelen B, van der Vlist M, Boekhout T, Geijtenbeek TB
    Nat. Immunol., 2012-01-22;13(3):246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
    Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
    J. Leukoc. Biol., 2011-06-07;90(3):599-611.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  31. Selective C-Rel activation via Malt1 controls anti-fungal T(H)-17 immunity by dectin-1 and dectin-2.
    Authors: Gringhuis SI, Wevers BA, Kaptein TM, van Capel TM, Theelen B, Boekhout T, de Jong EC, Geijtenbeek TB
    PLoS Pathog., 2011-01-20;7(1):e1001259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  32. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
    Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
    Immunol. Lett., 2010-10-07;134(2):167-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Modulatory role of vitamin A on the Candida albicans-induced immune response in human monocytes
    Authors: Tilman E Klassert, Anja Hanisch, Julia Bräuer, Esther Klaile, Kerstin A Heyl, Michael K Mansour et al.
    Medical Microbiology and Immunology
  34. Dectin-1 Is Expressed in Human Lung and Mediates the Proinflammatory Immune Response to Nontypeable Haemophilus influenzae
    Authors: Kerstin A. Heyl, Tilman E. Klassert, Annina Heinrich, Mario M. Müller, Esther Klaile, Hendrik Dienemann et al.
    mBio

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Dectin-1/CLEC7A Antibody

Average Rating: 5 (Based on 3 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Dectin-1/CLEC7A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Dectin-1/CLEC7A Antibody
By Anonymous on 01/16/2024
Application: Flow

Detection of human Dectin1 on HEK cells overexpressing isoform A of human Dectin1. The cells were treated with 100 nM of the human Dectin1 (catalogue # MAB1859) (BLUE) or mouse IgG2b isotype control antibody (RED) followed by treatments with a secondary goat anti-mouse IgG AF647-conjugated antibody

Flow Cytometry Human Dectin-1/CLEC7A Antibody MAB1859
Human Dectin-1/CLEC7A Antibody
By Anonymous on 11/28/2020
Application: Flow Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Dectin1 is detected in THP1 monocytes using 100 nM of the Human Dectin-1/CLEC7A Antibody clone 259931. The 259931 antibody was detected with a secondary goat anti-mouse IgG Fc Alexa Fluor 647 antibody.

Flow Cytometry Human Dectin-1/CLEC7A Antibody MAB1859
Human Dectin-1/CLEC7A Antibody
By Anonymous on 10/13/2020
Application: Flow Sample Tested: macrophages Species: Human

Detection of human Dectin­1 in monocyte-derived cultured macrophages by Flow Cytometry. Human monocytes were cultured in MCSF for 6 days to differentiate into macrophages. After differentiation, the macrophages were stained with the mouse anti-human Dectin­1 antibody clone 259931 at 100 nM or the mouse IgG2b isotype control. Binding was detected with a secondary goat a-mouse IgG Fcγ fragment APC antibody.

Flow Cytometry Human Dectin-1/CLEC7A Antibody MAB1859