Home / E-Cadherin / Human E-Cadherin PE-conjugated Antibody

Human E-Cadherin PE-conjugated Antibody

Catalog #: FAB18381P
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB18381P-100
FAB18381P-025
Detection of E-Cadherin in MCF-7 Human Cell Line by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Cadherin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human E-Cadherin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 180224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Asp155-Ile707
Accession # P12830
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of E-Cadherin antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of E-Cadherin in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human E-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB18381P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Human E-Cadherin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human Renal Normal, Tumoral, and Cancer Stem Cells Express Membrane-Bound Interleukin-15 Isoforms Displaying Different Functions1
    Authors: Sandy Azzi, Cindy Gallerne, Cristina Romei, Vincent Le Coz, Rosaria Gangemi, Krystel Khawam et al.
    Neoplasia
  2. BTG2 loss and miR-21 upregulation contribute to prostate cell transformation by inducing luminal markers expression and epithelial–mesenchymal transition
    Authors: V Coppola, M Musumeci, M Patrizii, A Cannistraci, A Addario, M Maugeri-Saccà et al.
    Oncogene
  3. Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro
    Authors: Subayyil, AA;Basmaeil, YS;Kulayb, HB;Alrodayyan, M;Alhaber, LAA;Almanaa, TN;Khatlani, T;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cell-based selection provides novel molecular probes for cancer stem cells.
    Authors: Sefah K, Bae K, Phillips J, Siemann D, Su Z, McClellan S, Vieweg J, Tan W
    Int J Cancer, 2013-02-08;132(11):2578-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012-02-21;7(2):e31624.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human embryonic stem cells with maintenance under a feeder-free and recombinant cytokine-free condition.
    Authors: Nakahara M, Saeki K, Nakamura N
    Cloning Stem Cells, 2009-03-01;11(1):5-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Amygdalin inhibits the growth of renal cell carcinoma cells in vitro
    Authors: EVA JUENGEL, ANITA THOMAS, JOCHEN RUTZ, JASMINA MAKAREVIC, IGOR TSAUR, KAREN NELSON et al.
    International Journal of Molecular Medicine
  8. Infection and transmission of SARS‐CoV‐2 depend on heparan sulfate proteoglycans
    Authors: Marta Bermejo‐Jambrina, Julia Eder, Tanja M Kaptein, John L van Hamme, Leanne C Helgers, Killian E Vlaming et al.
    The EMBO Journal

FAQs

  1. Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    • The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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