E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.
Human E‑Selectin/CD62E Biotinylated Antibody
R&D Systems | Catalog # BBA8
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # BBIG-E5
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Product Specifications
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human E‑Selectin/CD62E Biotinylated Antibody
E-Selectin/CD62E in HUVEC Cells.
E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Applications for Human E‑Selectin/CD62E Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF alpha
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF alpha
Western Blot
1 µg/mL
Sample: Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
under non-reducing conditions only
Sample: Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
under non-reducing conditions only
Human E-Selectin/CD62E Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BBA8 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: E-Selectin/CD62E
Alternate Names
CD62E, ELAM1, LECAM2, SELE
Gene Symbol
SELE
Additional E-Selectin/CD62E Products
Product Documents for Human E‑Selectin/CD62E Biotinylated Antibody
Product Specific Notices for Human E‑Selectin/CD62E Biotinylated Antibody
For research use only
Citations for Human E‑Selectin/CD62E Biotinylated Antibody
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: endothelial cellsSpecies: HumanVerified Customer | Posted 10/14/2022
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars