What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?

Name: Human EGFR (Research Grade Cetuximab Biosimilar) PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB9577P
Price: 439 USD

Catalog #s MAB9577 and FAB9577 have a kappa light chain.

Human EGFR (Research Grade Cetuximab Biosimilar) PE-conjugated Antibody

Catalog #: FAB9577P Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
FAB9577P-100

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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

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Human EGFR (Research Grade Cetuximab Biosimilar) PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGFR based on Cetuximab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Cetuximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu1

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human EGF R/ErbB1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) PE‑conjugated Monoclonal Antibody (Catalog # FAB9577P, filled histogram) or unstained cells (open histogram).View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: EGFR

Cetuximab is a biosimilar directed against the extracellular domain of EGFR. This product can be used in flow cytometry or various assay format to measure EGFR expression.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?
- Catalog #s MAB9577 and FAB9577 have a kappa light chain.

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