Human EMILIN2 Antibody

Catalog #: MAB10590 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10590-100
MAB10590-SP

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Human EMILIN2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EMILIN2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1031401

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human EMILIN2 synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed THP‑1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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EMILIN2 in THP‑1 Human Cell Line. EMILIN2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Mouse Anti-Human EMILIN2 Monoclonal Antibody (Catalog # MAB10590) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMILIN2

EMILIN (Elastin Microfibril Interface Located Protein) is an elastic fiber-associated glycoprotein. EMILIN2 is a protein that may be responsible for anchoring smooth muscles to elastin fibers. It also may be involved in the process that regulates vessel assembly. MRNA expression of EMILIN2 is more restricted compared to EMILIN1 with highest levels being present in fetal heart and adult lung. EMILIN2 is secreted extracellularly by in vitro-grown cells.

Long Name

Elastin Microfibril Interfacer 2

Entrez Gene IDs

84034 (Human)

Alternate Names

Elastin microfibril interface-located protein 2; elastin microfibril interfacer 2EMILIN-2; EMIL2; EMILIN2; extracellular glycoprotein EMILIN-2; FLJ33200; FOAP-10; Protein FOAP-10