Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human EMMPRIN/CD147 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
9.77 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human EMMPRIN
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human EMMPRIN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human EMMPRIN in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human EMMPRIN and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human EMMPRIN showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally ocurring EMMPRIN.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 448 | 1257 | 2412 | 396 | 1183 | 2363 |
| Standard Deviation | 20.1 | 43 | 120 | 21.1 | 70.5 | 120 |
| CV% | 4.5 | 3.4 | 5 | 5.3 | 6 | 5.1 |
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 431 | 1208 | 2341 | 421 | 1240 | 2474 |
| Standard Deviation | 15.3 | 46.9 | 109 | 23.7 | 54.9 | 145 |
| CV% | 3.5 | 3.9 | 4.7 | 5.6 | 4.4 | 5.9 |
Recovery
The recovery of EMMPRIN spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 91-111 |
| EDTA Plasma (n=4) | 98 | 90-110 |
| Heparin Plasma (n=4) | 96 | 85-104 |
| Serum (n=4) | 100 | 90-111 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of EMMPRIN were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
N/A
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Human EMMPRIN/CD147 ELISA Cell Culture Supernates/Saliva/Urine/Human Milk Standard Curve
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EMMPRIN/CD147
The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.
Long Name:
Extracellular Matrix Metalloproteinase Inducer
Entrez Gene IDs:
682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)
Alternate Names:
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human EMMPRIN/CD147 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Factors Associated with RANTES, EMMPIRIN, MMP2 and MMP9, and the Association of These Biomarkers with Cardiovascular Disease in a Multi-Ethnic Population
Authors: LY Wang, CS Tan, MKP Lai, S Hilal
Journal of Clinical Medicine, 2022-12-08;11(24):.
Species: Human
Sample Types: Serum
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Cluster of Differentiation 147 (CD147) serves as a promoter of atherosclerosis in patients with cerebral infarction
Authors: GJ Wang, X Dong, Y Li
European review for medical and pharmacological sciences, 2022-08-01;26(16):5710-5717.
Species: Human
Sample Types: Serum
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BSG (CD147) Serum Level and Genetic Variants Are Associated with Overall Survival in Acute Myeloid Leukaemia
Authors: P ?acina, A Butrym, E Turlej, M Stachowicz, J Wietrzyk, G Mazur, K Bogunia-Ku
Journal of Clinical Medicine, 2022-01-10;11(2):.
Species: Human
Sample Types: Serum
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CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults
Authors: MM Ali, I Mirza, D Naquiallah, C Hassan, M Masrur, FM Bianco, AM Mahmoud
Journal of cardiovascular development and disease, 2021-12-28;9(1):.
Species: Human
Sample Types: Plasma
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Clinical Significance of CD147 in Children with Inflammatory Bowel Disease
Authors: H Wang, J Ye, R Liu, G Chen, J Zhao, L Huang, F Yang, M Li, S Zhang, Jingxie, L Xiong, H Chen, Y Xu, M Su, Y Xie, S Li, F Zheng, L Geng, W Xu, S Gong
Biomed Res Int, 2020-09-15;2020(0):7647181.
Species: Human
Sample Types: Serum
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Soluble EMMPRIN levels discriminate aortic ectasia in Marfan syndrome patients
Authors: E Rurali, GL Perrucci, R Gaetano, A Pini, D Moschetta, D Gentilini, P Nigro, G Pompilio
Theranostics, 2019-04-12;9(8):2224-2234.
Species: Human
Sample Types: Plasma
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Transmembrane Protease TMPRSS11B Promotes Lung Cancer Growth by Enhancing Lactate Export and Glycolytic Metabolism
Authors: BL Updegraff, X Zhou, Y Guo, MS Padanad, PH Chen, C Yang, J Sudderth, C Rodriguez-, L Girard, JD Minna, P Mishra, RJ DeBerardin, KA O'Donnell
Cell Rep, 2018-11-20;25(8):2223-2233.e6.
Species: Human
Sample Types: Cell Culture Supernates
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Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma.
Authors: Wu J, Hao Z, Zhao Y, Yang X, Tang H, Zhang X, Song F, Sun X, Wang B, Nan G, Chen Z, Bian H
J Transl Med, 2014-07-04;12(0):190.
Species: Human
Sample Types: Serum
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Human EMMPRIN/CD147 Quantikine ELISA Kit
By Marry Tahani
on 11/21/2018
Sample Tested: Serum
