Human ERK1 Antibody

Catalog #: AF1879 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1879
AF1879-SP

Detection of Human ERK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ERK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERK1 in Western blots. Does not detect recombinant or endogenous human ERK2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ERK1
Ala2-Pro379
Accession # P27361

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ERK1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1879) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1

ERK1 (also known as MAPK3, Mitogen-activated protein kinase 3) is a 44 kDa phosphoprotein. It is Serine/threonine proteinkinase which acts as an essential component of the MAP kinase signal transduction pathway. ERK1 was initially isolated and cloned as a kinase activated in response to insulin and NGF. It is expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activates human ERK1 at Thr202/Tyr204. Within the range used as an immunogen human and mouse ERK1 share 97% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Alternate Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24