Human ETV1 Antibody

Catalog #: MAB9389 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9389-100
MAB9389-SP

Detection of Human ETV1 by Western Blot.

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All ETV1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ETV1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ETV1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 815207

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ETV1
Asn120-Leu190
Accession # P50549

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ETV1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ETV1 Monoclonal Antibody (Catalog # MAB9389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ETV1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ETV1

ETS translocation variant 1 is a protein that in humans is encoded by the ETV1 gene. ETV-1, ETV-4 and ETV-5 proteins form the PEA3 subfamily of ETS transcription factors. Posttranslational modifications in this subfamily promote cell proliferation, motility and invasion. Disregulation of ETV-1 has been observed in gastrointestinal stromal and protatic tumors, and in triple-negative breast cancer. Human ETV-1 is a 477 aminoacids (aa) protein with at least six known isoforms. Over the immunogen used in this antibody (aa120-190), ETV-1 sequences are 96% identical to mouse and rat ETV-1 sequences.

Long Name

ETS Translocation Variant 1

Entrez Gene IDs

2115 (Human); 14009 (Mouse); 362733 (Rat)

Alternate Names

DKFZp781L0674; ER81; ER81ets variant gene 1; ETS translocation variant 1; ets variant 1; Ets-related protein 81; ETV1; MGC104699; MGC120533; MGC120534