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Human FABP5/E-FABP Antibody

Catalog #: MAB3077
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3077
MAB3077-SP
Detection of Human FABP5/E‑FABP by Western Blot.
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP5/E-FABP Antibody Summary

Species Reactivity
Human
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed.
Source
Monoclonal Rat IgG2A Clone # 311215
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
HUVEC human umbilical vein endothelial cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Knockout Validated
FABP5/E‑FABP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in FABP5/E‑FABP knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FABP5/E-FABP antibody by Western Blot. View Larger

Detection of Human FABP5/E‑FABP by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry FABP5 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

FABP5 in HUVEC Human Cells. FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-con-jugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human FABP5/E-FABP Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human FABP5/E‑FABP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/E-FABP at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP5/E-FABP

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is expressed in skin, lens, adipose tissue, endothelial cells, heart, brain and placenta. FABP-5 is associated with keratinocytes and adipocytes, and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors.  Human FABP-5 shares 80%, 81%, and 92% aa identity with mouse, rat and bovine FABP-5, respectively.

Long Name
Fatty Acid-Binding Protein 5
Entrez Gene IDs
2171 (Human); 16592 (Mouse); 140868 (Rat)
Alternate Names
EFABP; E-FABP; E-FABPPAFABP; FABP5; fatty acid binding protein 5 (psoriasis-associated); Fatty acid-binding protein 5; PA-FABPepidermal; Psoriasis-associated fatty acid-binding protein homolog

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Citations for Human FABP5/E-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. CRABP2 and FABP5 expression levels in diseased and normal pancreas
    Authors: CS Hughes, JA ChinAleong, HM Kocher
    Ann Diagn Pathol, 2020-06-20;47(0):151557.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cigarette smoke inhibits LPS-induced FABP5 expression by preventing c-Jun binding to the FABP5 promoter
    Authors: D Rao, AL Perraud, C Schmitz, F Gally
    PLoS ONE, 2017-05-18;12(5):e0178021.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue
    Authors: Wentao Chen, Jiajia Dong, Lars Plate, David E. Mortenson, Gabriel J. Brighty, Suhua Li et al.
    Journal of the American Chemical Society
  4. Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma
    Authors: YOSHITO OHYAMA, YUKIHIRO KAWAMOTO, TADASHIGE CHIBA, KENTARO KIKUCHI, HIDEAKI SAKASHITA, KAZUSHI IMAI
    Molecular and Clinical Oncology
  5. Cigarette Smoke Decreases Airway Epithelial FABP5 Expression and Promotes Pseudomonas aeruginosa Infection
    Authors: Fabienne Gally, Hong Wei Chu, Russell P. Bowler
    PLoS ONE
  6. Molecular determinants of retinoic acid sensitivity in pancreatic cancer.
    Authors: Gupta S, Pramanik D, Mukherjee R, Campbell NR, Elumalai S, de Wilde RF, Hong SM, Goggins MG, De Jesus-Acosta A, Laheru D, Maitra A
    Clin. Cancer Res., 2011-10-18;18(1):280-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  7. Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1.
    Authors: Liebertz DJ, Lechner MG, Masood R
    Head Neck Oncol, 2010-02-22;2(0):5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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