Home / Fc gamma RII/CD32 / Human Fc gamma  RII/CD32 Antibody

Human Fc gamma  RII/CD32 Antibody

Catalog #: AF1875
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1875
AF1875-SP
Detection of Fc gamma  RII/CD32 in Whole blood granulocytes by Flow Cytometry
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Citations (12)
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Human Fc gamma  RII/CD32 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fc gamma RIIIB is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RIIA/CD32a
Ala36-Ile218
Accession # AAA35827
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Fc gamma  RIIA/CD32a (Catalog # 1330-CD)
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood granulocytes
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of human IgG to immobilized Recombinant Human Fc gamma RIIA/CD32a (Catalog # 1330-CD) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Fc gamma  RII/CD32 in Whole blood granulocytes by Flow Cytometry Whole blood granulocytes were stained with Goat Anti-Human Fc gamma  RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1875, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/CD32

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1 - 3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a
high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1 - 3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.
Long Name
Fc gamma Receptor II
Entrez Gene IDs
2212 (Human); 14130 (Mouse)
Alternate Names
CD32 antigen; CD32B; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RII; Fc gamma RIIb; FCG2; Fc-gamma RII-b; FcgammaRIIb; fc-gamma-RIIb; FCGR2; FcgRII; FcgRIIB; fcRII-b; FcRII-c; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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Citations for Human Fc gamma  RII/CD32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis
    Authors: Tomas Castro-Dopico, Thomas W. Dennison, John R. Ferdinand, Rebeccah J. Mathews, Aaron Fleming, Dean Clift et al.
    Immunity
  2. A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples
    Authors: Margaret E. Ackerman, Brian Moldt, Richard T. Wyatt, Anne-Sophie Dugast, Elizabeth McAndrew, Stephen Tsoukas et al.
    Journal of Immunological Methods
  3. Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells
    Authors: LA Henze, TTD Luong, B Boehme, J Masyout, MP Schneider, S Brachs, F Lang, B Pieske, A Pasch, KU Eckardt, J Voelkl, I Alesutan
    Aging (Albany NY), 2019-08-03;11(15):5445-5462.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Antibody-dependent and -independent uptake of HBsAg across human leukocyte subsets is similar between individuals with chronic hepatitis B Virus infection and healthy donors
    Authors: Hugo Tharinger
    J. Viral Hepat, 2017-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification.
    Authors: Tjiam M, Taylor J, Morshidi M, Sariputra L, Burrows S, Martin J, Deeks S, Tan D, Lee S, Fernandez S, French M
    J Immunol, 2015-04-24;194(11):5320-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. FcgammaRI mediates serum amyloid P inhibition of fibrocyte differentiation.
    Authors: Crawford J, Pilling D, Gomer R
    J Leukoc Biol, 2012-04-09;92(4):699-711.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Nonfucosylated anti-CD20 antibody potentially induces apoptosis in lymphoma cells through enhanced interaction with FcgammaRIIIb on neutrophils.
    Authors: Nakagawa T, Natsume A, Satoh M, Niwa R
    Leuk. Res., 2010-05-01;34(5):666-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Association of FcgammaRIIa (CD32a) with lipid rafts regulates ligand binding activity.
    Authors: Bournazos S, Hart SP, Chamberlain LH, Glennie MJ, Dransfield I
    J. Immunol., 2009-06-15;182(12):8026-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. The ubiquitin ligase c-Cbl down-regulates FcgammaRIIa activation in human neutrophils.
    Authors: Marois L, Vaillancourt M, Marois S, Proulx S, Pare G, Rollet-Labelle E, Naccache PH
    J. Immunol., 2009-02-15;182(4):2374-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008-06-30;84(4):1109-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
    Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
    Atherosclerosis, 2007-05-24;196(1):129-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function.
    Authors: Zhang R, Becnel L, Li M, Chen C, Yao Q
    Eur. J. Immunol., 2006-11-01;36(11):2993-3006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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