Human FCRL6/FcRH6 Antibody

Catalog #: AF6284 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6284
AF6284-SP

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Detection of Human FCRL6/FcRH6 by Western Blot.

1 Image

All FCRL6/FcRH6 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRL6/FcRH6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRL6/FcRH6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FcRH1, rhFcRH2, and rhFcRH4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FCRL6/FcRH6
Lys16-Leu312
Accession # Q6DN72

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Customers also Viewed

Human Galectin-7 Antibody

Cat # MAB13391

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human FCRL6/FcRH6 by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FCRL6/FcRH6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRL6/FcRH6 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily. It is found on CD56dim CD16⁺ NK cells, CD56⁺ CD3⁺ NKT cells, gd T cells, and effector plus effector-memory CD8⁺ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II. Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein. It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434. Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

Long Name

Fc Receptor-like 6

Entrez Gene IDs

343413 (Human); 677296 (Mouse)

Alternate Names

Fc receptor-like 6; FcRH6; FCRL6; FLJ16056; IFGP6