Human FCRL6/FcRH6 Antibody

Catalog #: AF6284 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6284
AF6284-SP

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Detection of Human FCRL6/FcRH6 by Western Blot.

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All FCRL6/FcRH6 Antibodies

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Human FCRL6/FcRH6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRL6/FcRH6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FcRH1, rhFcRH2, and rhFcRH4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FCRL6/FcRH6
Lys16-Leu312
Accession # Q6DN72

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FCRL6/FcRH6 by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FCRL6/FcRH6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRL6/FcRH6 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily. It is found on CD56dim CD16⁺ NK cells, CD56⁺ CD3⁺ NKT cells, gd T cells, and effector plus effector-memory CD8⁺ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II. Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein. It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434. Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

Long Name

Fc Receptor-like 6

Entrez Gene IDs

343413 (Human); 677296 (Mouse)

Alternate Names

Fc receptor-like 6; FcRH6; FCRL6; FLJ16056; IFGP6