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Human FGF acidic/FGF1 Antibody

Catalog #: AF232
13 Citations Datasheet / COA / SDS
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AF232
AF232-SP
Detection of Human FGF acidic/FGF1 by Western Blot.
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Citations (13)
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Human FGF acidic/FGF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse FGF acidic/FGF1 is observed. Neutralizes the biological activity of rhFGF acidic/FGF1. It will also neutralize the biological activity of rh beta -ECGF and bovine FGF acidic/FGF1, although 2‑3 times the amount of IgG is required to neutralize bovine FGF acidic/FGF1 biological activity.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FGF acidic/FGF1
Phe16-Asp155
Accession # P05230
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize FGF acidic/FGF1-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically < 2 µg/mL in the presence of 0.75 ng/mL Recombinant Human FGF acidic/FGF1 aa 16‑155 and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FGF acidic/FGF1 antibody by Western Blot. View Larger

Detection of Human FGF acidic/FGF1 by Western Blot. Western blot shows lysates of human brain (hypothalamas) tissue and human heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF acidic/FGF1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FGF acidic/FGF1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

FGF acidic/FGF1 in Human Breast. FGF acidic/FGF1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Human FGF acidic/FGF1 Antibody. View Larger

Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Human FGF acidic/FGF1 Antibody. Recombinant Human FGF acidic/FGF1 aa 16-155 (Catalog # 232-FA) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF acidic/FGF1 aa 16-155 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232). The ND50 is typically < 2 µg/mL in the presence of heparin (10 µg/mL).

Simple Western Detection of Human FGF acidic/FGF1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human FGF acidic/FGF1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.2 mg/mL. A specific band was detected for FGF acidic/FGF1 at approximately 25 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence Coculture increases proliferation of me-CH. (a) BrdU was stained for proliferating cells at day 3. Positive cells are shown in red, indicated by white arrowheads. Green cells are PKH67 labeled me-CH. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (N = 3). Data is shown as mean + standard deviation. Statistical significance was analyzed by Student's t-test. **P < 0.01. (c) Immunofluorescent and BrdU staining for FGF1 is performed on SSCs pellet and coculture pellet. Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25852755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence Conditioned medium of SSCs increases proliferation of me-CH through FGF1 signaling pathway. (a) BrdU was stained for proliferating cells at day 3 after forming of aggregates. Pellets of me-CH were cultured in SF medium (serum free medium plus 5 μg/mL normal goat IgG), Con medium (conditioned medium plus 5 μg/mL normal goat IgG), or Con medium + anti-FGF1 (conditioned medium plus 5 μg/mL of goat antibody against FGF1). Positive cells are shown in red, indicated by white arrowheads. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (N = 3). Data is shown as mean + standard deviation. Statistical significance was analyzed by one-way ANOVA followed by Dunnett's test. *P < 0.05. **P < 0.01. (c) Immunofluorescent staining for FGF1 is performed on SSCs (passage 2). Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25852755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence Conditioned medium of SSCs increases proliferation of me-CH through FGF1 signaling pathway. (a) BrdU was stained for proliferating cells at day 3 after forming of aggregates. Pellets of me-CH were cultured in SF medium (serum free medium plus 5 μg/mL normal goat IgG), Con medium (conditioned medium plus 5 μg/mL normal goat IgG), or Con medium + anti-FGF1 (conditioned medium plus 5 μg/mL of goat antibody against FGF1). Positive cells are shown in red, indicated by white arrowheads. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (N = 3). Data is shown as mean + standard deviation. Statistical significance was analyzed by one-way ANOVA followed by Dunnett's test. *P < 0.05. **P < 0.01. (c) Immunofluorescent staining for FGF1 is performed on SSCs (passage 2). Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25852755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Human FGF acidic/FGF1 by Immunocytochemistry/ Immunofluorescence Coculture increases proliferation of me-CH. (a) BrdU was stained for proliferating cells at day 3. Positive cells are shown in red, indicated by white arrowheads. Green cells are PKH67 labeled me-CH. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (N = 3). Data is shown as mean + standard deviation. Statistical significance was analyzed by Student's t-test. **P < 0.01. (c) Immunofluorescent and BrdU staining for FGF1 is performed on SSCs pellet and coculture pellet. Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25852755), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF acidic/FGF1

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).

References
  1. Jaye, M. et al. (1986) Science 233:541.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.
  5. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  6. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  7. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  8. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  9. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  10. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  11. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  12. Imamura, T. et al. (1990) Science 249:1567.
  13. Wesche, J. et al. (2005) Biochemistry 44:6071.
  14. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  15. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  16. Bouleau, S. et al. (2005) Oncogene 24:7839.
Long Name
Fibroblast Growth Factor acidic
Entrez Gene IDs
2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
Alternate Names
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1

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Citations for Human FGF acidic/FGF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer
    Authors: Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al.
    Journal of Experimental Medicine
  2. Tumor-derived Immunoglobulin-like transcript 4 facilitates angiogenesis of colorectal cancer
    Authors: J Liu, F Zhang, J He, S Wang, L Wang, J Li, W Shi, Y Han, A Gao, Y Sun
    American journal of cancer research, 2023-02-15;13(2):419-435.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer
    Authors: HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith
    British Journal of Cancer, 2022-07-01;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells
    Authors: A Lampart, KD Sluzalska, A Czyrek, A Szerszen, J Otlewski, A Wiedlocha, M Zakrzewska
    Cells, 2022-02-02;11(3):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.
  6. Pituitary Adenylate Cyclase-Activating Peptide (PACAP), a Novel Secretagogue, Regulates Secreted Morphogens in Newborn Rat Retina
    Authors: M Lakk, V Denes, K Kovacs, O Hideg, BF Szabo, R Gabriel
    Invest. Ophthalmol. Vis. Sci, 2017-01-01;58(1):565-572.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Beneficial effects of coculturing synovial derived mesenchymal stem cells with meniscus fibrochondrocytes are mediated by fibroblast growth factor 1: increased proliferation and collagen synthesis.
    Authors: Song, Xuanhe, Xie, Yaoping, Liu, Yang, Shao, Ming, Wang, Wenbo
    Stem Cells Int, 2015-03-16;2015(0):926325.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  8. WNT7A/beta-catenin signaling induces FGF1 and influences sensitivity to niclosamide in ovarian cancer.
    Authors: King M, Lindberg M, Stodden G, Okuda H, Ebers S, Johnson A, Montag A, Lengyel E, MacLean Ii J, Hayashi K
    Oncogene, 2014-09-01;34(26):3452-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Reduction of brain metastases in plasminogen activator inhibitor-1-deficient mice with transgenic ocular tumors.
    Authors: Maillard CM, Bouquet C, Petitjean MM, Mestdagt M, Frau E, Jost M, Masset AM, Opolon PH, Beermann F, Abitbol MM, Foidart JM, Perricaudet MJ, Noel AC
    Carcinogenesis, 2008-08-27;29(11):2236-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Molecular profiling of cervical cancer progression.
    Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
    Br. J. Cancer, 2007-01-29;96(2):321-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005-02-16;132(6):1223-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. FGF1 C-terminal domain and phosphorylation regulate intracrine FGF1 signaling for its neurotrophic and anti-apoptotic activities
    Authors: E Delmas, N Jah, C Pirou, S Bouleau, N Le Floch, J-L Vayssière et al.
    Cell Death & Disease
  13. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms.
    Authors: Haichuan H, Zofia P, Patricia H et al.
    Cancer Cell.

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