Human Fibroblast Activation Protein alpha /FAP APC-conjugated Antibody
Human Fibroblast Activation Protein alpha /FAP APC-conjugated Antibody Summary
Leu26-Asp760
Accession # Q12884
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Fibroblast Activation Protein alpha /FAP in WI‑38 Human Cell Line by Flow Cytometry. WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP APC-conjugated Monoclonal Antibody (Catalog # FAB3715A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Fibroblast Activation Protein alpha /FAP in U87MG cells by Flow Cytometry. U87MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein alpha /FAP APC‑conjugated Monoclonal Antibody (Catalog # FAB3715A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fibroblast Activation Protein alpha/FAP
FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin alpha 3 beta 1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.
- Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
- Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
- Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
- O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
- Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
- Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
Product Datasheets
Citations for Human Fibroblast Activation Protein alpha /FAP APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Cancer-associated fibroblasts derived fibronectin extra domain A promotes sorafenib resistance in hepatocellular carcinoma cells by activating SHMT1
Authors: Dong, Y;Chen, Y;Wang, Y;Zhao, X;Zi, R;Hao, J;Ding, Q;Jiang, H;Wang, X;Lu, F;Liang, H;Wei, Z;Li, J;
Genes & diseases
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines
Authors: MR Ambrosio, G Mosca, T Migliaccio, D Liguoro, G Nele, F Schonauer, F D'Andrea, F Liotti, N Prevete, RM Melillo, C Reale, C Ambrosino, C Miele, F Beguinot, V D'Esposito, P Formisano
Cancers, 2022-11-03;14(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment
Authors: Z Pan, T Xu, L Bao, X Hu, T Jin, J Chen, J Chen, Y Qian, X Lu, L Li, G Zheng, Y Zhang, X Zou, F Song, C Zheng, L Jiang, J Wang, Z Tan, P Huang, M Ge
Molecular Cancer, 2022-10-03;21(1):190.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC -
IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies
Authors: M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie
Nature Medicine, 2021-10-21;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
LncRNA MIR31HG is induced by tocilizumab and ameliorates rheumatoid arthritis fibroblast-like synoviocyte-mediated inflammation via miR-214-PTEN-AKT signaling pathway
Authors: Liang Cao, Haifeng Jiang, Jing Yang, Jun Mao, Guofeng Wei, Xiangyun Meng et al.
Aging (Albany NY)
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Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of Tumors
Authors: Vinit Kumar, Laxminarasimha Donthireddy, Douglas Marvel, Thomas Condamine, Fang Wang, Sergio Lavilla-Alonso et al.
Cancer Cell
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Other experimental details provided in:
Lindeman, Spencer D., et al. Fibroblast Activation Protein–Targeted Radioligand Therapy for Treatment of Solid Tumors. Journal of Nuclear Medicine 64.5 (2023): 759-766.
https://doi.org/10.2967/jnumed.122.264494
