Human Frizzled-7 Antibody Summary
Gln33-Leu185
Accession # NP_003498
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Frizzled-7 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Sheep Anti-Human Frizzled-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6178, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-7
Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1-3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N-terminal signal peptide (amino acid (aa) 1-32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44-169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257-549), and a variable-length C-terminal cytoplasmic domain (aa 550-574) (1-3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33-185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta -Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3-5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).
- Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
- Vincan, E. et al. (2007) Front. Biosci. 12:4558.
- Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
- Medina, A. et al. (2000) Mech. Dev. 92:227.
- Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
- Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
- Melchior, K. et al. (2008) Biol. Chem. 389:897.
- Vincan, E. et al. (2007) Oncogene 26:2340.
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