Human Galectin-3 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 0.78 | 2.27 | 5.06 | 0.81 | 2.51 | 5.29 |
Standard Deviation | 0.03 | 0.1 | 0.15 | 0.07 | 0.2 | 0.36 |
CV% | 3.8 | 4.4 | 3 | 8.6 | 8 | 6.8 |
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (ng/mL) | 0.79 | 2.46 | 5.11 | 0.8 | 2.59 | 5.5 |
Standard Deviation | 0.03 | 0.09 | 0.18 | 0.05 | 0.16 | 0.32 |
CV% | 3.8 | 3.7 | 3.5 | 6.3 | 6.2 | 5.8 |
Recovery
The recovery of Galectin-3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 102 | 96-106 |
EDTA Plasma (n=4) | 105 | 93-113 |
Heparin Plasma (n=4) | 103 | 95-110 |
Serum (n=4) | 104 | 94-115 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Galectin-3
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Galectin-3 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation
Authors: Kosmider, O;Possémé, C;Templé, M;Corneau, A;Carbone, F;Duroyon, E;Breillat, P;Chirayath, TW;Oules, B;Sohier, P;Luka, M;Gobeaux, C;Lazaro, E;Outh, R;Le Guenno, G;Lifermann, F;Berleur, M;Le Mene, M;Friedrich, C;Lenormand, C;Weitten, T;Guillotin, V;Burroni, B;Boussier, J;Willems, L;Aractingi, S;Dionet, L;Tharaux, PL;Vergier, B;Raynaud, P;Ea, HK;Ménager, M;Duffy, D;Terrier, B;
Nature communications
Species: Human
Sample Types: Plasma
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Prognostic Value of Galectin-3 after Left Atrial Appendage Occlusion for Predicting Peri-Device Leakage
Authors: Haertel, F;Lustermann, P;Hamadanchi, A;Gruen, K;Bogoviku, J;Aftanski, P;Westphal, J;Baez, L;Franz, M;Schulze, PC;Moebius-Winkler, S;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Increased Levels of Galectin-3 in Critical COVID-19
Authors: Nikitopoulou, I;Vassiliou, AG;Athanasiou, N;Jahaj, E;Akinosoglou, K;Dimopoulou, I;Orfanos, SE;Dimakopoulou, V;Schinas, G;Tzouvelekis, A;Aidinis, V;Kotanidou, A;
International journal of molecular sciences
Species: Human
Sample Types: Serum
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Factors associated with patent foramen ovale-related stroke: SAFAS study
Authors: Pommier, T;Lafont, A;Didier, R;Garnier, L;Duloquin, G;Meloux, A;Sagnard, A;Graber, M;Dogon, G;Laurent, G;Vergely, C;Béjot, Y;Guenancia, C;
Revue neurologique
Species: Human
Sample Types: Serum
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Identification of protein biomarkers associated with congenital diaphragmatic hernia in human amniotic fluid
Authors: Bhutada, S;Tran-Lundmark, K;Kramer, B;Conner, P;Lowry, AM;Blackstone, E;Frenckner, B;Mesas-Burgos, C;Apte, SS;
Scientific reports
Species: Human
Sample Types: Amniotic Fluid
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Galectin-3 as a Marker for Increased Thrombogenicity in COVID-19
Authors: Puccini, M;Jakobs, K;Reinshagen, L;Friebel, J;Schencke, PA;Ghanbari, E;Landmesser, U;Haghikia, A;Kr�nkel, N;Rauch, U;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Fibrosis of Peritoneal Membrane, Molecular Indicators of Aging and Frailty Unveil Vulnerable Patients in Long-Term Peritoneal Dialysis
Authors: P Branco, R Calça, AR Martins, C Mateus, MJ Jervis, DP Gomes, S Azeredo-Lo, AF De Melo Ju, C Sousa, E Civantos, S Mas-Fontao, A Gaspar, S Ramos, J Morello, F Nolasco, A Rodrigues, SA Pereira
International Journal of Molecular Sciences, 2023-03-06;24(5):.
Species: Human
Sample Types: Serum
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Plasma galectin-3 concentration and estimated glomerular filtration rate in patients with type 2 diabetes with and without albuminuria
Authors: JO Chung, SY Park, SB Lee, NR Kang, DH Cho, DJ Chung, MY Chung
Scientific Reports, 2022-09-29;12(1):16328.
Species: Human
Sample Types: Plasma
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The interaction between rs 3,807,992 genotypes with the dietary inflammatory index on Leptin, Leptin resistance, and Galectin 3 in obese and overweight women
Authors: F Shiraseb, M Farazi, N Rasaei, CCT Clark, S Jamili, K Mirzaei
BMC endocrine disorders, 2022-09-23;22(1):237.
Species: Human
Sample Types: Serum
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Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study
Authors: J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk
International Journal of Environmental Research and Public Health, 2022-09-13;19(18):.
Species: Human
Sample Types: Serum
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Effects of liraglutide vs. lifestyle changes on soluble suppression of tumorigenesis-2 (sST2) and galectin-3 in obese subjects with prediabetes or type 2 diabetes after comparable weight loss
Authors: P Simeone, R Tripaldi, A Michelsen, T Ueland, R Liani, S Ciotti, KI Birkeland, HL Gulseth, A Di Casteln, F Cipollone, P Aukrust, A Consoli, B Halvorsen, F Santilli
Cardiovascular Diabetology, 2022-03-11;21(1):36.
Species: Human
Sample Types: Serum
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The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation
Authors: A Rühle, AL Grosu, N Wiedenmann, J Ruf, B Bieber, R Stoian, AR Thomsen, E Gkika, P Vaupel, D Baltas, WA Weber, M Mix, NH Nicolay
Clinical and translational radiation oncology, 2022-02-21;33(0):120-127.
Species: Human
Sample Types: Plasma
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Therapeutic implications of galectin-3 in patients with atrial fibrillation
Authors: KN Lee, DY Kim, KY Boo, YG Kim, SY Roh, YS Baek, DH Kim, DI Lee, J Shim, JI Choi, GS Hwang, YH Kim
Scientific Reports, 2022-01-17;12(1):784.
Species: Human
Sample Types: Serum
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Serum Galectin-3 levels and all-cause and cardiovascular mortality in maintenance hemodialysis patients: a prospective cohort study
Authors: S Liu, Q Wu, S Zhang, Z Wang, H Liu, L Teng, P Xiao, Y Lu, X Wang, C Dong, J Xiao, J Zhang
Bmc Nephrology, 2022-01-03;23(1):5.
Species: Human
Sample Types: Serum
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Diagnostic Significance of Serum Galectin-3 in Hospitalized Patients with COVID-19-A Preliminary Study
Authors: B Ku?nierz-C, B Maziarz, P Dumnicka, M Dembi?ski, M Kapusta, M Boci?ga-Ja, M Winiarski, A Garlicki, T Grodzicki, M Kukla
Oncogene, 2021-08-01;11(8):.
Species: Human
Sample Types: Serum
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Analysis of the Correlation of Galectin-3 Concentration with the Measurements of Echocardiographic Parameters Assessing Left Atrial Remodeling and Function in Patients with Persistent Atrial Fibrillation
Authors: P Wa?ek, U Grabowska, E Cie?la, J Sielski, J Roskal-Wa?, B Wo?akowska
Biomolecules, 2021-07-28;11(8):.
Species: Human
Sample Types: Serum
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The immunoregulatory function of peripheral blood CD71+ erythroid cells in systemic-onset juvenile idiopathic arthritis
Authors: H Kanemasa, M Ishimura, K Eguchi, T Tanaka, E Nanishi, A Shiraishi, M Goto, Y Motomura, S Ohga
Scientific Reports, 2021-07-13;11(1):14396.
Species: Human
Sample Types: Serum
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Myocardial extracellular volume quantification by computed tomography predicts outcomes in patients with severe aortic stenosis
Authors: Y Hammer, Y Talmor-Bar, A Abelow, K Orvin, Y Aviv, N Bar, A Levi, U Landes, G Shafir, A Barsheshet, H Vaknin-Ass, A Sagie, R Kornowski, A Hamdan
PLoS ONE, 2021-03-10;16(3):e0248306.
Species: Human
Sample Types: Serum
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Adaptive response triggered by the repeated SCUBA diving is reflected in cardiovascular, muscular, and immune biomarkers
Authors: M Žarak, A Perovi?, M Njire Brat, S Šupraha Go, J Dumi?
Physiological Reports, 2021-01-01;9(2):e14691.
Species: Human
Sample Types: Plasma
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Galactin-1, 3 and 9: Potential biomarkers in idiopathic pulmonary fibrosis and other interstitial lung diseases
Authors: M d'Alessand, E De Vita, L Bergantini, MA Mazzei, S di Valvaso, M Bonizzoli, A Peris, P Sestini, E Bargagli, D Bennett
Respir Physiol Neurobiol, 2020-09-11;282(0):103546.
Species: Human
Sample Types: Serum
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Galectin-3 enhances neutrophil motility and extravasation into the airways during Aspergillus fumigatus�infection
Authors: BD Snarr, G St-Pierre, B Ralph, M Lehoux, Y Sato, A Rancourt, T Takazono, SR Baistrocch, R Corsini, MP Cheng, M Sugrue, LR Baden, K Izumikawa, H Mukae, JR Wingard, IL King, M Divangahi, MS Satoh, BG Yipp, S Sato, DC Sheppard
PLoS Pathog., 2020-08-04;16(8):e1008741.
Species: Human
Sample Types: Serum
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Systemic right ventricle in elderly patients with congenitally corrected transposition of the great arteries: Clinical profile, cardiac biomarkers, and echocardiographic parameters
Authors: E Kowalik, A Kwiatek-Wr, A Klisiewicz, A Lutynska, EK Biernacka, M Kowalski, P Hoffman
Anatol J Cardiol, 2020-08-01;24(2):92-96.
Species: Human
Sample Types: Plasma
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Galectin-3 as the Prognostic Factor of Adverse Cardiovascular Events in Long-Term Follow up in Patients after Myocardial Infarction-A Pilot Study
Authors: P ?wi?cki, R Sawicki, M Knapp, KA Kami?ski, K Ptaszy?ska, B Sobkowicz, A Lisowska
J Clin Med, 2020-05-29;9(6):.
Species: Human
Sample Types: Serum
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Evaluation of Inflammation Caused by Cardiopulmonary Bypass in a Small Animal Model
Authors: Y Fujii
Biology (Basel), 2020-04-20;9(4):.
Species: Rat
Sample Types: Plasma
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Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants
Authors: YH Jung, D Han, SH Shin, EK Kim, HS Kim
Sci Rep, 2020-03-04;10(1):4057.
Species: Human
Sample Types: Urine
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Systemic Inflammation Precedes Microalbuminuria in Diabetes
Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
Kidney Int Rep, 2019-06-21;4(10):1373-1386.
Species: Human
Sample Types: Serum
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Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
J Clin Med, 2018-11-08;7(11):.
Species: Human
Sample Types: Plasma
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Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
J Am Heart Assoc, 2018-11-06;7(21):e009098.
Species: Human
Sample Types: Plasma
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Prognostic Utility of Soluble Suppression of Tumorigenicity 2 level as a Predictor of Clinical Outcomes in Incident Hemodialysis Patients
Authors: SM Seo, SH Kim, Y Kim, HE Yoon, SJ Shin
Int J Med Sci, 2018-05-14;15(7):730-737.
Species: Human
Sample Types: Plasma
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Galectin-3 and incident cognitive impairment in REGARDS, a cohort of blacks and whites
Authors: A Venkatrama, P Callas, LA McClure, F Unverzagt, G Arora, V Howard, VG Wadley, M Cushman, P Arora
Alzheimers Dement (N Y), 2018-04-26;4(0):165-172.
Species: Human
Sample Types: Serum
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Work Intensity, Low-Grade Inflammation, and Oxidative Status: A Comparison between Office and Slaughterhouse Workers
Authors: S Zelzer, F Tatzber, M Herrmann, W Wonisch, S Rinnerhofe, M Kundi, B Obermayer-, T Niedrist, G Cvirn, G Wultsch, H Mangge
Oxid Med Cell Longev, 2018-04-18;2018(0):2737563.
Species: Human
Sample Types: Serum
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Evaluation of the association between serum uric acid level andthe predicted risk score of sudden cardiac death in five years in patients with hypertrophic cardiomyopathy
Authors: S Özy?lmaz, MH Sat?lm??o?, M Gül, H Uyarel, OA Serdar
Turk Kardiyol Dern Ars, 2018-03-01;46(2):111-120.
Species: Human
Sample Types: Serum
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The prognostic value of sST2 and galectin-3 considering different aetiologies in non-ischaemic heart failure
Authors: D Binas, H Daniel, A Richter, V Ruppert, KD Schlüter, B Schieffer, S Pankuweit
Open Heart, 2018-02-26;5(1):e000750.
Species: Human
Sample Types: Serum
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High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach
Authors: A Salah, H Yoshifuji, S Ito, K Kitagori, K Kiso, N Yamada, T Nakajima, H Haga, T Tsuruyama, A Miyagawa-H
Patholog Res Int, 2017-05-16;2017(0):9312142.
Species: Human
Sample Types: Serum
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Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
Hellenic J Cardiol, 2017-03-28;0(0):.
Species: Human
Sample Types: Serum
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Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
Sci Rep, 2017-01-23;7(0):40994.
Species: Human
Sample Types: Serum
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Aldosterone induced galectin-3 secretion in vitro and in vivo: from cells to humans.
Authors: Lin Y, Chou C, Wu X, Chang Y, Hung C, Chen Y, Tzeng Y, Wu V, Ho Y, Hsieh F, Wu K
PLoS ONE, 2014-09-02;9(9):e95254.
Species: Human
Sample Types: Cell Culture Supernates
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Galectin-3 in cord blood of term and preterm infants.
Authors: Demmert M, Faust K, Bohlmann MK, Troger B, Gopel W, Herting E, Hartel C
Clin. Exp. Immunol., 2012-02-01;167(2):246-51.
Species: Human
Sample Types: Cell Culture Supernates
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Hypoxia potentiates glioma-mediated immunosuppression.
Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
PLoS ONE, 2011-01-20;6(1):e16195.
Species: Human
Sample Types: Cell Culture Supernates
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