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Human Galectin-9 Quantikine ELISA Kit

Catalog #: DGAL90
17 Citations 1 Review Datasheet / COA / SDS
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DGAL90
Control Products Available
QC96: Quantikine Immunoassay Control Set 792 for Human Galectin-9
Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve
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Product Details
Procedure
Citations (17)
FAQs
Supplemental Products
Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Galectin-9 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (100 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
0.028 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human Galectin-9.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Galectin-9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Galectin-9 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Galectin-9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Galectin-9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Galectin-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Saliva, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.34 3.58 6.13 1.44 3.43 6.05
Standard Deviation 0.05 0.12 0.22 0.08 0.18 0.32
CV% 3.7 3.4 3.6 5.6 5.2 5.3

Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.44 3.77 6.29 1.4 3.28 5.84
Standard Deviation 0.06 0.07 0.08 0.11 0.2 0.3
CV% 4.2 1.9 1.3 7.9 6.1 5.1

Recovery

The recovery of human Galectin-9 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 84-104
EDTA Plasma (n=4) 103 85-117
Heparin Plasma (n=4) 104 89-117
Serum (n=4) 106 86-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Galectin-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Galectin-9 ELISA Linearity

Scientific Data

N/A Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve View Larger

Human Galectin-9 ELISA Serum/Plasma/Urine Standard Curve

N/A Human Galectin-9 ELISA Cell Culture Supernate/Saliva/Milk Standard Curve View Larger

Human Galectin-9 ELISA Cell Culture Supernate/Saliva/Milk Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-9

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3965 (Human); 16859 (Mouse)
Alternate Names:
Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. PROTECT FROM LIGHT.
  9.   For Cell Culture Supernate, Saliva, or Human Milk Samples: Incubate at room temperature for 20 minutes on the benchtop.
    For Serum, Plasma, or Urine Samples: Incubate at room temperature for 30 minutes on the benchtop.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Galectin-9 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. An NFAT1-C3a-C3aR Positive Feedback Loop in Tumor-Associated Macrophages Promotes a Glioma Stem Cell Malignant Phenotype
    Authors: Zhang, Y;Song, Y;Wang, X;Shi, M;Lin, Y;Tao, D;Han, S;
    Cancer immunology research
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Do Serum Galectin-9 Levels in Women with Gestational Diabetes and Healthy Ones Differ before or after Delivery? A Pilot Study
    Authors: Pe?ech, A;Rusza?a, M;Niebrzydowska-Tatus, M;Bie?, K;Kimber-Trojnar, ?;Czuba, M;?wistowska, M;Leszczy?ska-Gorzelak, B;
    Biomolecules
    Species: Human
    Sample Types: Serum, Urine
  3. Increased Galectin-9 Levels Correlate with Disease Activity in Patients with DMARD-Na�ve Rheumatoid Arthritis and Modulate the Secretion of MCP-1 and IL-6 from Synovial Fibroblasts
    Authors: MA Nielsen, D Køster, AY Mehta, K Stengaard-, P Busson, P Junker, K Hørslev-Pe, ML Hetland, M Østergaard, M Hvid, H Leffler, TW Kragstrup, RD Cummings, B Deleuran
    Cells, 2023-01-15;12(2):.
    Species: Human
    Sample Types: Plasma
  4. Clinical and serological association of plasma 25-hydroxyvitamin D (25(OH)D) levels in lupus and the short-term effects of oral vitamin D supplementation
    Authors: C Kavadichan, P Singh, S Maurya, S Tota, A Kiroubagar, D Kounassega, S Anand, VS Negi, A Aggarwal
    Arthritis Research & Therapy, 2023-01-03;25(1):2.
    Species: Human
    Sample Types: Plasma
  5. Serum Levels of Galectin-9 are Increased in Cervical Cancer Patients and are Higher in Advanced Clinical Stages
    Authors: T Reyes-Vall, I Conde-Rodr, J Serna-Vill, I Ramírez-Dí, G Pérez-Vill, G Delgado-Ló, VJ Vazquez-Za, CT Gutiérrez-, L Ávila-Jimé, A García-Car, L Martínez-A, G Santos-Lóp, J Reyes-Leyv, V Vallejo-Ru
    OncoTargets and Therapy, 2022-10-13;15(0):1211-1220.
    Species: Human
    Sample Types: Serum
  6. Association between inflammatory cytokines and immune-checkpoint molecule in rheumatoid arthritis
    Authors: H Matsumoto, Y Fujita, T Asano, N Matsuoka, J Temmoku, S Sato, M Yashiro-Fu, K Yokose, S Yoshida, E Suzuki, T Yago, H Watanabe, A Kawakami, K Migita
    PLoS ONE, 2021-11-18;16(11):e0260254.
    Species: Human
    Sample Types: Serum
  7. LINC00261 elevation inhibits angiogenesis and cell cycle progression of pancreatic cancer cells by upregulating SCP2 via targeting FOXP3
    Authors: J Zou, X Pei, D Xing, X Wu, S Chen
    Journal of Cellular and Molecular Medicine, 2021-09-19;0(0):.
    Species: Human
    Sample Types: Serum
  8. Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis
    Authors: LL Eriksen, MA Nielsen, TL Laursen, B Deleuran, H Vilstrup, S Støy
    PLoS ONE, 2021-08-05;16(8):e0255574.
    Species: Human
    Sample Types: Plasma
  9. Galactin-1, 3 and 9: Potential biomarkers in idiopathic pulmonary fibrosis and other interstitial lung diseases
    Authors: M d'Alessand, E De Vita, L Bergantini, MA Mazzei, S di Valvaso, M Bonizzoli, A Peris, P Sestini, E Bargagli, D Bennett
    Respir Physiol Neurobiol, 2020-09-11;282(0):103546.
    Species: Human
    Sample Types: Serum
  10. Detection of pancreatic ductal adenocarcinoma with galectin-9 serum levels
    Authors: AM Seifert, C Reiche, M Heiduk, A Tannert, AC Meinecke, S Baier, J von Reness, C Kahlert, M Distler, T Welsch, C Reissfelde, DE Aust, G Miller, J Weitz, L Seifert
    Oncogene, 2020-02-13;0(0):.
    Species: Human
    Sample Types: Serum
  11. Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization
    Authors: X Zhang, D He, S Gao, Y Wei, L Wang
    Mol Med Rep, 2019-06-06;20(2):1241-1249.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Clinical Significance of Serum Galectin-9 and Soluble CD155 Levels in Patients with Systemic Sclerosis
    Authors: M Chihara, M Kurita, Y Yoshihara, A Asahina, K Yanaba
    J Immunol Res, 2018-11-27;2018(0):9473243.
    Species: Human
    Sample Types: Serum
  13. Expression profiles and clinical value of plasma exosomal Tim-3 and Galectin-9 in non-small cell lung cancer
    Authors: J Gao, X Qiu, X Li, H Fan, F Zhang, T Lv, Y Song
    Biochem. Biophys. Res. Commun., 2018-02-13;0(0):.
    Species: Human
    Sample Types: Plasma
  14. TIM-3 plays a more important role than PD-1 in the functional impairments of cytotoxic T cells of malignant Schwannomas
    Authors: Z Li, X Liu, R Guo, P Wang
    Tumour Biol., 2017-05-01;39(5):1010428317698.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
    Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
    Sci Rep, 2017-01-23;7(0):40994.
    Species: Human
    Sample Types: Serum
  16. Increased peripheral cd4+ regulatory t cells persist after successful direct-acting antiviral treatment of chronic hepatitis C
    Authors: Bettina Langhans
    J. Hepatol, 2016-12-29;0(0):.
    Species: Human
    Sample Types: Serum
  17. Human Galectin-9 Is a Potent Mediator of HIV Transcription and Reactivation
    Authors: Mohamed Abdel-Mohs
    PLoS Pathog, 2016-06-02;12(6):e1005677.
    Species: Human
    Sample Types: Plasma

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Human Galectin-9 Quantikine ELISA Kit
By Adam Guess on 06/21/2016
Sample Tested: Mesenchymal stem cells
Human Galectin-9 Quantikine ELISA Kit DGAL90