Human gamma-Synuclein Antibody

Catalog #: AF5745 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5745
AF5745-SP

Detection of Human gamma -Synuclein by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human gamma-Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human gamma -Synuclein in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human
beta -Synuclein is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human gamma -Synuclein
Asn47-Glu120
Accession # O76070

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human gamma -Synuclein by Western Blot. Western blot shows lysates of human brain tissue. PVDF Membrane was probed with 1 µg/mL of Human gamma-Synuclein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5745) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

Immunohistochemistry

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gamma -Synuclein in Human Cerebellum. gamma-Synuclein was detected in immersion fixed paraffin-embedded sections of human cerebellum using Human gamma-Synuclein Antigen Affinity Purified Polyclonal Antibody (Catalog # AF5745) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Entrez Gene IDs

6623 (Human)

Alternate Names

BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma