Human GATA-6 Antibody

Catalog #: MAB1700
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1700
MAB1700-SP

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All GATA-6 Antibodies

Product Details

Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GATA-6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) GATA-1 or rhGATA-2.

Source

Monoclonal Mouse IgG₁ Clone # 222228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # CAA64997

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human GATA‑6

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GATA‑6 in HCT‑116 Human Cell Line. GATA-6 was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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GATA‑6 in HT‑29 Human Cell Line. GATA-6 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GATA-6 Monoclonal Antibody (Catalog # MAB1700) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-6

GATA-6 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-6 is expressed in visceral and parietal endoderm and other tissues. It interacts with several additional proteins to regulate gene expression in heart, lung, gut and gonads (1).

References

Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Entrez Gene IDs

2627 (Human)

Alternate Names

GATA binding protein 6; GATA6; GATA-6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human GATA-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk
Authors: Yu, L;Logsdon, D;Pinzon-Arteaga, CA;Duan, J;Ezashi, T;Wei, Y;Ribeiro Orsi, AE;Oura, S;Liu, L;Wang, L;Liu, K;Ding, X;Zhan, L;Zhang, J;Nahar, A;Stobbe, C;Katz-Jaffe, M;Schoolcraft, WB;Tan, T;Hon, GC;Yuan, Y;Wu, J;
Cell stem cell
Species: Human
Sample Types: Whole Cells
Applications: ICC
Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs
Authors: RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai
International Journal of Molecular Sciences, 2022-06-30;23(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Self-organization of the human embryo in the absence of maternal tissues
Nat. Cell Biol., 2016-05-04;18(6):700-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo.
Authors: Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M
Proc. Natl. Acad. Sci. U.S.A., 2010-03-22;107(14):6364-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Heparan sulfation-dependent fibroblast growth factor signaling maintains embryonic stem cells primed for differentiation in a heterogeneous state.
Authors: Lanner F, Lee KL, Sohl M, Holmborn K, Yang H, Wilbertz J, Poellinger L, Rossant J, Farnebo F
Stem Cells, 2010-02-01;28(2):191-200.
Species: Human
Sample Types: Whole Cells
Applications: ICC