Human GATA-6 Biotinylated Antibody

Catalog #: BAF1700 Datasheet / COA / SDS

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BAF1700

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Human GATA-6 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-6.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GATA-6
Met1-Ala449
Accession # Q92908-2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GATA‑6

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GATA‑6 in KATO-III Human Cell Line. GATA‑6 was detected in immersion fixed KATO-III human gastric carcinoma cell line using 10 µg/mL Human GATA‑6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1700) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Streptavidin (red, upper panel; Catalog # NL999) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATA-6

GATA-6 (also named GATA-GT1) is a zinc finger transcription activating protein that bins to a T/A-G-A-T-A-G/A DNA sequence motif in mesodermally and endodermally-derived tissue. There are two GATA-6 isoforms that arise from alternate start sites: a short form of 449 amino acids (aa), and a long form of 595 aa. The short form of human GATA-6 is 93%, 92% and 88% aa identical to GATA-6 in porcine, rat and mouse, respectively.

Entrez Gene IDs

2627 (Human)

Alternate Names

GATA binding protein 6; GATA6; GATA-6; GATA-binding factor 6; GATA-binding protein 6; transcription factor GATA-6