Human GFAP Antibody

Detection of GFAP in Human Brain Cortex via seqIF™ staining on COMET™​ GFAP Antibody was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 0.25ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.​​

Detection of Human GFAP by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (motor cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GFAP in U-251MG Human Cell Line. GFAP was detected in immersion fixed U-251MG human glioblastoma cell line using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GFAP in Human Brain. GFAP was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 0.2 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

GFAP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. GFAP was detected in immersion fixed U937 human histiocytic lymphoma cell line but is not detected in GFAP knockout (KO) U937 Human cell line using Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human GFAP by Simple Western^TM. Simple Western lane view shows lysates of human cerebellum, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 45-55 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GFAP Monoclonal Antibody (Catalog # MAB25941). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.