Human GLI-2 Biotinylated Antibody

Catalog #: BAF3526 Datasheet / COA / SDS

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BAF3526

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Human GLI-2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GLI-2 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse GLI-2 is observed and 5% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GLI-2
Ala2-Glu91
Accession # BAA03568

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GLI‑2

Immunocytochemistry

5-15 µg/mL

Immersion fixed MDA-MB-231 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GLI‑2 in MDA‑MB‑231 Human Cell Line. GLI-2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Sheep Anti-Human GLI-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3526) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-2

GLI-2 is a 166 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least four isoforms that differ in their N- or C-terminal regions have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, human and mouse GLI-2 share 88% amino acid sequence homology.

Long Name

GLI-Kruppel family member GLI2

Entrez Gene IDs

2736 (Human); 14633 (Mouse); 304729 (Rat)

Alternate Names

GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2