Human HE4/WFDC2 Antibody

Catalog #: MAB62741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB62741-100
MAB62741-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HE4/WFDC2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HE4/WFDC2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 676009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HE4/WFDC2
Thr28-Phe124
Accession # Q14508

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human HE4/WFDC2 DuoSet ELISA Kit (Catalog # DY6274-05) for convenient development of a sandwich ELISA or the Human HE4/WFDC2 Quantikine ELISA Kit (Catalog # DHE400) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human HE4/WFDC2 ELISA Standard Curve. Recombinant Human HE4/WFDC2 protein was serially diluted 2-fold and captured by Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB62741) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human HE4/WFDC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6274) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HE4/WFDC2

HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Ovarian epithelial tumors have an increased expression of HE4, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.

Long Name

WAP Four-disulfide Core Domain protein 2

Entrez Gene IDs

10406 (Human); 67701 (Mouse); 286888 (Rat)

Alternate Names

dJ461P17.6; EDDM4; Epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2