Human Hexim 1 Antibody

Catalog #: AF8106 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8106
AF8106-SP

Detection of Human Hexim 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Hexim 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Hexim 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Hexim 2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Hexim 1
His213-Asp359
Accession # O94992

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Hexim 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hexim 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Hexim 1 in Human Liver. Hexim 1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Hexim 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8106) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hexim 1

Hexim 1, also known as CLP1, EDG1, HIS1, and MAQ1, is an approximately 60 kDa transcriptional regulator that binds double stranded RNA and inhibits RNA polymerase II. Hexim 1 plays an important role in many processes including satellite muscle cell regeneration, cardiovascular development, and HIV-1 replication. It can form homodimers and heterodimers with Hexim 2, and its activity is regulated by phosphoylation at Ser158. Hexim 1 inhibits the kinase activity of the elongation factor P-TEFb. This inhibition can be relieved by Hexim 1 interaction with Nucleophosphmin or by cellular exposure to hexamtehylene bisacetamide. Hexim 1 inhibits the transactivation function of the glucocorticoid, estrogen, and androgen receptors. It can inhibit the cellular response to hypoxia and promote cancer progression or, alternatively, can inhibit VEGF mediated angiogenesis and suppress metastasis. Within aa 213-359, human Hexim 1 shares 95% and 94% aa sequence identity with mouse and rat Hexim 1, respectively.

Long Name

Hexamethylene Bis-Acetamide Inducible 1

Entrez Gene IDs

10614 (Human); 192231 (Mouse); 498008 (Rat)

Alternate Names

Cardiac lineage protein 1; CLP1; CLP-1; CLP1hexamethylene-bis-acetamide-inducible transcript 1; EDG1HMBA-inducible; Estrogen down-regulated gene 1 protein; hexamethylene bis-acetamide inducible 1; Hexamethylene bis-acetamide-inducible protein 1; hexamthylene bis-acetamide inducible 1; Hexim 1; HEXIM1; HIS1; HIS1FLJ13562; HMBA-inducible; MAQ1; MAQ1hexamethylene bisacetamide-inducible protein; menage a quatre 1; Menage a quatre protein 1; protein HEXIM1