Human HIF-2 alpha /EPAS1 Antibody

Catalog #: MAB2886
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2886
MAB2886-SP

Detection of Human HIF‑2 alpha /EPAS1 by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HIF-2 alpha /EPAS1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HIF-2 alpha /EPAS1 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 318404

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HIF-2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line (HIF-2a positive), Caki-2 human clear cell carcinoma epithelial cell line (HIF-2a negative), and HeLa human cervical epithelial carcinoma cell line (HIF-2a negative). PVDF Membrane was probed with 1 µg/mL of Human HIF-2a/EPAS1 Monoclonal Antibody (Catalog # MAB2886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HIF-2a/EPAS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha

Long Name

Hypoxia-inducible Transcription Factor 2 alpha

Entrez Gene IDs

2034 (Human); 13819 (Mouse)

Alternate Names

Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human HIF-2 alpha /EPAS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The von Hippel-Lindau Chuvash mutation promotes pulmonary hypertension and fibrosis in mice.
Authors: Hickey MM, Richardson T, Wang T, Mosqueira M, Arguiri E, Yu H, Yu QC, Solomides CC, Morrisey EE, Khurana TS, Christofidou-Solomidou M, Simon MC
J. Clin. Invest., 2010-02-08;120(3):827-39.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot