Human HSP70/HSPA1A DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: HSP70/HSPA1A
The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.
Citations for Human HSP70/HSPA1A DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
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Heat Shock Protein 70 Constitutes a Promising Novel Biomarker in Differential Diagnosis between Takotsubo Syndrome and Non-ST-Segment Elevation Myocardial Infarction
Authors: Demirel, O;Paar, V;Tolnai, P;Topf, A;Hoppe, UC;Lichtenauer, M;Mirna, M;
Journal of clinical medicine
Species: Human
Sample Types: Serum
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Differential Expression of Circulating Damage-Associated Molecular Patterns in Patients with Coronary Artery Ectasia
Authors: Tsoporis, JN;Triantafyllis, AS;Kalogeropoulos, AS;Izhar, S;Rigopoulos, AG;Rallidis, LS;Sakadakis, E;Toumpoulis, IK;Salpeas, V;Leong-Poi, H;Parker, TG;Rizos, I;
Biomolecules
Species: Human
Sample Types: Plasma
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Alterations of the 70�kDa heat shock protein (HSP70) and sequestosome-1 (p62) in women with breast cancer
Authors: T Orfanelli, S Giannopoul, E Zografos, A Athanasiou, AM Bongiovann, G Doulaveris, TA Moo, D LaPolla, CN Bakoyianni, GE Theodoropo, GC Zografos, E Andreopoul, SS Witkin
Scientific Reports, 2021-11-15;11(1):22220.
Species: Human
Sample Types: Cell Culture Supernates
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A tumor-intrinsic PD-L1-NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy
Authors: B Theivanthi, KS Evans, NC DeVito, MP Plebanek, M Sturdivant, LP Wachsmuth, AK Salama, Y Kang, D Hsu, JM Balko, DB Johnson, M Starr, AB Nixon, A Holtzhause, BA Hanks
J. Clin. Invest., 2020-05-01;0(0):.
Species: Human
Sample Types: Plasma
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Vaginal Biomarkers That Predict Cervical Length and Dominant Bacteria in the Vaginal Microbiomes of Pregnant Women
Authors: SS Witkin, AF Moron, BJ Ridenhour, E Minis, A Hatanaka, SGP Sarmento, MS Franca, FHC Carvalho, TK Hamamoto, R Mattar, E Sabino, IM Linhares, MVC Rudge, LJ Forney
MBio, 2019-10-22;10(5):.
Species: Human
Sample Types: Vaginal Fluid
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Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
J Immunol Res, 2019-04-11;2019(0):1845128.
Species: Human
Sample Types: Serum
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Increased Plasma Levels of Danger-Associated Molecular Patterns Are Associated With Immune Suppression and Postoperative Infections in Patients Undergoing Cytoreductive Surgery and Hyperthermic Intraperitoneal Chemotherapy
Authors: GP Leijte, H Custers, J Gerretsen, A Heijne, J Roth, T Vogl, GJ Scheffer, P Pickkers, M Kox
Front Immunol, 2018-04-05;9(0):663.
Species: Human
Sample Types: Plasma
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Genotoxic stress modulates the release of exosomes from multiple myeloma cells capable of activating NK cell cytokine production: Role of HSP70/TLR2/NF-kB axis
Authors: E Vulpis, F Cecere, R Molfetta, A Soriani, C Fionda, G Peruzzi, G Caracciolo, S Palchetti, L Masuelli, L Simonelli, U D'Oro, MP Abruzzese, MT Petrucci, MR Ricciardi, R Paolini, M Cippitelli, A Santoni, A Zingoni
Oncoimmunology, 2017-01-13;6(3):e1279372.
Species: Human
Sample Types: Cell Lysates
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We run human plasma samples at an 1:2 dilution for maximum precision. Samples could be run at a maximum 1:5 dilution with reduced precision, but decreased loss of sample volume,
